南京师大学报（自然科学版）2006，Vol.29Issue(3)：91-94,4.
物种特异性PCR在中药材白花蛇舌草鉴定中的应用
Application of Species-specific PCR in the Identification of Hedyotis diffusa Willd
摘要
Abstract
Hedyotis diffusa Willd is an important traditional Chinese medicine but its adulteration is frequent. The aim of this paper is to provide a pair of the species-specific PCR primers and an effective method which may be used to identifyH. diffusa by the molecular biological measure.Hedyotis diffusa Willd and its adulterants are collected from the commercial markets. DNA sequences of the internal transcribed spacer (ITS) from 9 species are significantly different from one another. Based on rDNA ITS sequences of all samples, a pair of species-specific primers, B174 and B53, are designed to authenticateH. diffusa from the other species. The results indicate that a 207 bp DNA fragment is amplified fromH. diffusa, whereas no any fragment is amplified from the other 8 species under the same reaction condition. The primers designed in the present study are highly specific forH. diffusa. They could be used as key components in theH. diffusa identification kit.关键词
白花蛇舌草/物种特异性引物/鉴定Key words
Hedyotis diffusa Willd/Species-specific primer/Authentication分类
医药卫生引用本文复制引用
刘忠权,丁小余,丁秉中..物种特异性PCR在中药材白花蛇舌草鉴定中的应用[J].南京师大学报（自然科学版）,2006,29(3):91-94,4.基金项目
Supported by Foundation for Jiangsu Science and Technology Department (BS2001016). （BS2001016）
