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首页|期刊导航|中国海洋湖沼学报(英文版)|Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers

Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers

YAO Bing HU Xiaoli BAO Zhenmin LU Wei HU Jingjie

中国海洋湖沼学报(英文版)2007,Vol.25Issue(1):91-96,6.
中国海洋湖沼学报(英文版)2007,Vol.25Issue(1):91-96,6.

Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers

Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers

YAO Bing 1HU Xiaoli 1BAO Zhenmin 1LU Wei 1HU Jingjie1

作者信息

  • 1. Laboratory of Marine Genetics and Breeding MGB, Division of Life Science and Technology, Ocean University of China,Qingdao 266003, China
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摘要

Abstract

Sea cucumber Apostichopus japonicus samples were collected in Changdao, Penglai (PL),27 individuals, and Lingshandao, Qingdao (QD), 30 individuals, in the Shandong Peninsula, China. Ten SSR primers were used to assess the genetic variation and relationship between and within the two stocks.Respectively, for each stock, the percentage of polymorphic bands was 85.2% and 86.9%; the gene diversity was 0.360 5 and 0.342 8; and the Shannon's information index was 0.515 0 and 0.499 0. At species level, the percentage of polymorphic bands was 92.2%, the total gene diversity was 0.378 9 and the Shannon's information index was 0.550 4. The coefficient of overall genetic differentiation and the genetic distances between the stocks were also calculated to be 0.073 0 and 0.079 6 using the POPGENE program. Results show that the genetic diversity of the two stocks is still large but the genetic distance between the two stocks is close. A dendrogram was constructed for the 57 individuals from the two stocks,showing that the genetic structure was unitary for PL stock but complex for QD stock.

关键词

sea cucumber/Apostichopus japonicus/ISSR/stock/genetic diversity

Key words

sea cucumber/Apostichopus japonicus/ISSR/stock/genetic diversity

分类

生物科学

引用本文复制引用

YAO Bing,HU Xiaoli ,BAO Zhenmin,LU Wei,HU Jingjie..Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers[J].中国海洋湖沼学报(英文版),2007,25(1):91-96,6.

基金项目

Supported by the NSFC (No.30571417) and The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China (No.200426) (No.30571417)

中国海洋湖沼学报(英文版)

OACSCDCSTPCD

2096-5508

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