首页|期刊导航|中国海洋湖沼学报(英文版)|Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers
中国海洋湖沼学报(英文版)2007,Vol.25Issue(1):91-96,6.
Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers
Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers
摘要
Abstract
Sea cucumber Apostichopus japonicus samples were collected in Changdao, Penglai (PL),27 individuals, and Lingshandao, Qingdao (QD), 30 individuals, in the Shandong Peninsula, China. Ten SSR primers were used to assess the genetic variation and relationship between and within the two stocks.Respectively, for each stock, the percentage of polymorphic bands was 85.2% and 86.9%; the gene diversity was 0.360 5 and 0.342 8; and the Shannon's information index was 0.515 0 and 0.499 0. At species level, the percentage of polymorphic bands was 92.2%, the total gene diversity was 0.378 9 and the Shannon's information index was 0.550 4. The coefficient of overall genetic differentiation and the genetic distances between the stocks were also calculated to be 0.073 0 and 0.079 6 using the POPGENE program. Results show that the genetic diversity of the two stocks is still large but the genetic distance between the two stocks is close. A dendrogram was constructed for the 57 individuals from the two stocks,showing that the genetic structure was unitary for PL stock but complex for QD stock.关键词
sea cucumber/Apostichopus japonicus/ISSR/stock/genetic diversityKey words
sea cucumber/Apostichopus japonicus/ISSR/stock/genetic diversity分类
生物科学引用本文复制引用
YAO Bing,HU Xiaoli ,BAO Zhenmin,LU Wei,HU Jingjie..Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers[J].中国海洋湖沼学报(英文版),2007,25(1):91-96,6.基金项目
Supported by the NSFC (No.30571417) and The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China (No.200426) (No.30571417)