中华医学杂志（英文版）2004，Vol.117Issue(6)：922-926,5.
Influence of CaNa2 EDTA on topical 5-aminolaevulinic acid photodynamic therapy
Influence of CaNa2 EDTA on topical 5-aminolaevulinic acid photodynamic therapy
刘辉峰 1徐世正 1张春荣1
作者信息
- 1. Department of Dermatology, Renmin Hospital, Wuhan University, Wuhan 430060,China
- 折叠
摘要
Abstract
Background We asssessed whether the CaNa2 EDTA could improve the accumulation of protoporphyrin Ⅸ (PpⅨ) and photosensitisation in HEp-2 cells as well as the depth of treatment of skin cancers on the topical 5-Aminolaevulinic acid (5-ALA) PDT.Methods HEp-2 cells were incubated with 5-ALA (0-1mmol/L) and CaNa2EDTA (0-1mmol/L) for 4 hours, intracellular protoporphyrin Ⅸ content was quantified by extraction, and cell viability was assessed by use of the methyl-tetrazolium (MTT) assay four hours after exposure to light. In comparison with the pictures before and after treatment, depth of treatment could be determined using a Acuson Sequioa 512 phase-array system in paired experiments.Results PpⅨ accumulation increased with increasing extracellular concentrations of ALA (0-1mmol/L). Adding 1mmol/L of CaNa2EDTA increased 30% PpⅨ accumulation over the same period of incubation in the concentration of 1mmol/L ALA. Significant difference was observed between the 5-ALA alone group and 5-ALA combined CaNa2 EDTA group in the PpⅨ accumulation (P<0.01). Cell viability after exposure to light decreased with adding CaNa2 EDTA, a statistical difference in a same fluence above 1.2 J/cm2 between two groups was demonstrated (P<0.05, P<0.01 respectively). Depth of treatment of skin cancers were increased in CaNa2 EDTA-treated group.conclusion CaNa2 EDTA could improve the PpⅨ accumulation and photosensitisation in HEp-2 cells. Clinically, CaNa2 EDTA could increase the depth of treatment in the cutaneous cancers.关键词
photodynamic therapy (PDT)/iron chelator/5-Aminolaevulinic acid/cutaneous cancerKey words
photodynamic therapy (PDT)/iron chelator/5-Aminolaevulinic acid/cutaneous cancer分类
医药卫生引用本文复制引用
刘辉峰,徐世正,张春荣..Influence of CaNa2 EDTA on topical 5-aminolaevulinic acid photodynamic therapy[J].中华医学杂志（英文版）,2004,117(6):922-926,5.
