中华医学杂志（英文版）2002，Vol.115Issue(6)：923-927,5.
银染mRNA差异显示法分离黄芪当归肾脏保护作用相关基因
Identification of a gene associated with astragalus and angelica's renal protective effects by silver staining mRNA differential display
摘要
Abstract
Objective To identify genes associated with the chronic progression of renal disease and astragalus and angelica (A&A)'s renal protective effects.Methods The technique of silver staining mRNA differential display (DD) was used to investigate changes of gene expression in normal, sclerotic and A&A treated sclerotic kidneys. We isolated genes differentially expressed during the progression of renal disease which could be normalized by A&A.Results Several genes related to A&A's protective effects were isolated and one of them was confirmed by Northern blot.Conclusion Silver staining mRNA differential display is a simple and effective technique for isolating differentially expressed genes. The isolated new gene may be related to the progression of chronic renal disease and contribute to A&A's protective effects.关键词
肾脏/纤维化/基因表达/黄芪/当归Key words
kidney/fibrosis/gene expression/astragalus/angelica分类
医药卫生引用本文复制引用
余凌,李惊子,王海燕,吕有勇..银染mRNA差异显示法分离黄芪当归肾脏保护作用相关基因[J].中华医学杂志（英文版）,2002,115(6):923-927,5.基金项目
This work was supported in part by National Natural Science Foundation of China (No. 39970929). （No. 39970929）
