首页|期刊导航|中国农业科学（英文版）|Inhibitory Effects of Kojic Acid on Phenoloxidase of Diamondback Moth Plutella xylostella
中国农业科学（英文版）2004，Vol.3Issue(12)：923-930,8.
Inhibitory Effects of Kojic Acid on Phenoloxidase of Diamondback Moth Plutella xylostella
Inhibitory Effects of Kojic Acid on Phenoloxidase of Diamondback Moth Plutella xylostella
摘要
Abstract
The effects of kojic acid on phenoloxidase (PO) of Plutella xylostella were investigated, when it had been partially purified by 40% saturated [(NH4)2SO4] and Sephadex G-100 gel filtration. Kojic acid showed inhibitory effects on both monophenolase and o-diphenolase activity of the PO. The inhibitor concentrations leading to 50% (IC50) activity lost were estimated to be 0.07 mmol L-1 for monophenolase and 1 mmol L-1 for diphenolase, respectively. Kojic acid can also prolong the lag time of PO for oxidation of L-tyrosine. The inhibition kinetics were analyzed by Lineweaver-Burk plots and kojic acid was found to be a reversible competitive inhibitor with the Ki of 0.47 mmol L-1. The ability of kojic acid to inhibit the enzyme activity may be associated with its ability to chelate copper at the active site. In addition, the iron ion was found to shorten the lag time obviously, but have no important effect on the monophenolase activity.关键词
Kojic acid/Plutella xylostella/Phenoloxidase/Inhibitor/Inhibition kineticsKey words
Kojic acid/Plutella xylostella/Phenoloxidase/Inhibitor/Inhibition kinetics引用本文复制引用
WANG Shu-dong,LUO Wan-chun,GAO Xing-xiang,DING Qi..Inhibitory Effects of Kojic Acid on Phenoloxidase of Diamondback Moth Plutella xylostella[J].中国农业科学（英文版）,2004,3(12):923-930,8.基金项目
The study was supperted by the State Science and Technology Commission of China (30270887). （30270887）
