解放军医学杂志2001，Vol.26Issue(2)：92-95,4.
创伤后IL-2表达受抑与核转录因子NFAT变化的关系
THE DECLINE IN THE INDUCTION OF IL-2 EXPRESSION AFTER TRAUMA IS CORRELA-
TED TO CHANGES IN THE TRANSCRIPTION FACTOR NFAT
罗艳 1梁华平 1徐祥 1胡承香 1耿波 1王正国1
作者信息
摘要
Abstract
This study was designed to determine if the decrease in the expression of interleukin-2(IL-2) in spleen lymphocytes after trauma was associated with the changes in the transcription factor NFAT binding activity. Closed impact injury with fracture of both hind limbs in mice was adopted as the trauma model. Animals were sacrificed at the 12h and 1,4,7,10,14 days after injury. Spleen lymphocytes were isolated from traumatized mice and stimulated with concanavalin-A, The culture supernatants were harvested and assayed for IL-2 activity. Total RNA was extracted from spleen lymphocytes and assayed for IL-2 mRNA. Nuclear protein was extracted,and DNA binding activity of NFAT was measured using an electrophoretic mobility shift assay(EMSA). The results showed that the DNA binding activity of NFAT gradually decreased , with trough value of NFAT binding activity ,accounting for 41% of the control, observed at 4d after injury. It was closely associated with the decline of IL-2 activity and IL-2 mRNA. These data suggest that the decline in the induction of IL-2 expression is,at least, partially due to the impairment in the activation of NFAT in traumatized mice. Taken together, the results are significant to clarify the mechanism of the decline in the induction of IL-2 expression after trauma.关键词
创伤/白细胞介素2/IL-2 mRNA/NFAT/核转录因子分类
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罗艳,梁华平,徐祥,胡承香,耿波,王正国..创伤后IL-2表达受抑与核转录因子NFAT变化的关系[J].解放军医学杂志,2001,26(2):92-95,4.
