首页|期刊导航|高技术通讯（英文版）|T-DNA transfer and integration as a tool for insertional mutagenesis in the taxol-producing fungus
高技术通讯（英文版）2008，Vol.14Issue(1)：92-97,6.
T-DNA transfer and integration as a tool for insertional mutagenesis in the taxol-producing fungus
T-DNA transfer and integration as a tool for insertional mutagenesis in the taxol-producing fungus
摘要
Abstract
Agrobacterium tumefaciens-mediated DNA transformation method was applied to transform Nodulisporium sylviforme fusant HDF-68, a taxol-producing fungus. We constructed a binary vector pBI121-43 carrying a hygromycin-resistant gene cassette between the right and left borders of T-DNA. Optimal co-cultivation of N.sylviforme with A.tumefaciens containing pBI121-43 led to 110～130 hygromycin-resistant transformants per million conidia. Putative transformants were found to be mitotically stable. The molecular analysis of transformants demonstrated the random integration of single copy of the T-DNA into the host genome. This transformation system serves as a basic tool for insertional mutagenesis in N.sylviforme fusant HDF-68, and the development of such system lays a solid foundation for constructing high-yied gene engineering strain and clarifying taxol biosynthesis pathway in this fungus.关键词
taxol, T-DNA, insertional mutagenesisKey words
taxol, T-DNA, insertional mutagenesis分类
生物科学引用本文复制引用
Chi Yan,Ping Wenxiang,Li Shanshan,Zhu Jing,Ma Xi,Gao Fengshan,Zhou Dongpo..T-DNA transfer and integration as a tool for insertional mutagenesis in the taxol-producing fungus[J].高技术通讯（英文版）,2008,14(1):92-97,6.基金项目
Supported by the National Natural Science Foundation of China (No. 30570025) and the Education Department of Heilongjiang Province (No. 10551238). （No. 30570025）
