首页|期刊导航|中国农业科学（英文版）|Localization of Avian Influenza Virus in Formalin-Fixed, Paraffin-Embedded Chicken Tissues by In Situ Hybridization
中国农业科学（英文版）2005，Vol.4Issue(12)：931-936,6.
Localization of Avian Influenza Virus in Formalin-Fixed, Paraffin-Embedded Chicken Tissues by In Situ Hybridization
Localization of Avian Influenza Virus in Formalin-Fixed, Paraffin-Embedded Chicken Tissues by In Situ Hybridization
摘要
Abstract
In this study, in situ hybridization (ISH) was developed to detect avian influenza virus (AIV) in Madin-Darby canine kidney (MDCK) cells and formalin-fixed, paraffin-embedded chicken tissues. A cDNA probe corresponding to a region of AIV nucleoprotein (NP) gene was synthesized and labeled with digoxigenin. Probe specificity was determined by AIV infected MDCK cells in vitro and the results showed that strong cytoplasmic staining was only detected in AIV-infected cells. Various tissues were collected from 12 h to 3 5 days post-infection (PI) following inoculation with the H9N2 subtype AIV. AIV was localized in the epithelial cells of the duodenum and cartilage of the throat and trachea at 12 h PI. Tissues from uninfected chickens were negative. The finding of this study indicated ISH was a sensitive and specific technique to detect and localize AIV as well as to study AIV pathogenesis.关键词
In situ hybridization/Avian influenza virus/cDNA probe/DigoxigeninKey words
In situ hybridization/Avian influenza virus/cDNA probe/Digoxigenin分类
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ZHANG Wan-po,GU Chang-qin,BI Ding-ren,SONG Nian-hua,CHENG Guo-fu..Localization of Avian Influenza Virus in Formalin-Fixed, Paraffin-Embedded Chicken Tissues by In Situ Hybridization[J].中国农业科学（英文版）,2005,4(12):931-936,6.基金项目
The authors thank Dr. Liu Xianhong at Harvard Medical School for the review of the manuscript. Financial support was provided by the Science and Technology Commission of Hubei Province, China (2004AA202B04). （2004AA202B04）
