中国组织工程研究与临床康复2008，Vol.12Issue(47)：9367-9370,4.
人脐血源性间充质干细胞向心肌细胞的诱导分化
Differentiation of mesenchymal stem cells derived from human umbilical cord into cardiomyocytes
黄景玲 1杨水祥2
作者信息
- 1. 青海省心血管病专科医院心内科,青海省西宁市,810012
- 2. 北京世纪坛医院心内科,北京市,100038
- 折叠
摘要
Abstract
BACKGROUND:Compared with the bone marrow mesenchymal stem cells (BMSCs),cells from the umbilical cord blood (UCB)are considered"very young",and its proliferation and differentiation cannot decrease with age.Thus,cells from UCB are a great substitute of BMSCs.OBJECTIVE:To observe the characteristics of in vitro differentiation of UCB-MSCs into cardiomyocytes.DESIGN,TIME AND SETTING:The observational experiment was performed at the Beijing Sijitan Hospital from March 2005 to February 2007.MATERIALS:Cells from UCB were collected from healthy arturients after signing the informed consent.METHODS:Mononuclear cells were isolated to further harvest MSCs.At the third passage,CD34,CD44 and CD90 were measured using immunofluorescence flow cytometry.After 4 weeks of 5-azacitidine treatment,myosin heavy chain,GATA4 and troponin 1 were identified using immunohistochemistry and reverse transcription-polymerase chain reaction.MAIN OUTCOME MEASURES:β-myosin heavy chain,GATA4 and troponin I expression.RESULTS:UCB-MSCs showed a fibrolast-like morphology,clonally expanded after 5-azacitidine reatment.The immunophenotype of these clonally expanded cells is consistent with that reported for BMSCs.Immunohistochemistry and reverse transcription-polymerase chain reaction showed β-myosin heavy chain,GATA4 and troponin I expression.CONCLUSION:UCB-MSCs differentiate into cardiomyocyte-like cells,which have the characteristics of cardiomyocytes.关键词
间充质干细胞/心肌细胞/5-氮胞苷/脐血细胞分类
基础医学引用本文复制引用
黄景玲,杨水祥..人脐血源性间充质干细胞向心肌细胞的诱导分化[J].中国组织工程研究与临床康复,2008,12(47):9367-9370,4.
