安徽农业科学2009，Vol.37Issue(20)：9391-9392,9424,3.
CACNA2D1基因第14内含子的多态性及其对猪肌肉pH45的影响
Polymorphism of the 14th Intron of Porcine CACNA2D1 Gene and Influence on Muscular pH45
摘要
Abstract
[Objective]To detect polymorphism of the14th intron of porcine CACNA2D1 gene, and thus provide conditions for the marker-assisted selection of pork quality traits. [Method] The polymorphism of the14th exon and intron of porcine CACNA2D1 gene was detected in six breeds by PCR-SSCP. The genetic effects of different genotypes on the pH45 value of eye muscle and ham joint muscle were also analyzed. [Result] There were three kinds of genotypes, namely, AA, AG and GG, in the 14th intron of porcine CACNA2D1 gene. The polymorphic locus (G → A) was at the 1 145 nt of the sequence (GenBank Accepted No. FJ156361). The results of χ2 test showed that the distribution of these three kinds of genotypes was significantly different in the six breeds (P＜0.05). The pH45 values of eye muscle and ham joint muscle were significantly different between genotype AA and genotype AG of F2 generation from the Jianhua×Pietrain resource family (P＜0.05). [Conclusion] The polymorphism of the4th intron of porcine CACNA2D1 gene has effects on the pH45 values of eye muscle and ham joint muscle.关键词
猪/CACNA2D1基因/PCR-SSCP/pH45Key words
Pig/ CACNA2D1 gene/ PCR-SSCP/ pH45分类
农业科技引用本文复制引用
李建华,吕帮玉,杨明生..CACNA2D1基因第14内含子的多态性及其对猪肌肉pH45的影响[J].安徽农业科学,2009,37(20):9391-9392,9424,3.基金项目
湖北省教育厅优秀中青年人才项目(Q200626001). （Q200626001）
