中国农业科技导报2009，Vol.11Issue(3)：93-97,5.
拟南芥隐花色素突变体抑制子的筛选及其表型分析
Screening and Phenotypic Analysis of a Suppressor of Cryptochromes Mutant in Arabidopsis
摘要
Abstract
Cryptochromes are flavoproteins that share similarity with the sequence of amino acids in DNA photolyase but lack photolyase activity. Among members of cryptochromes gene family in Arabidopsis thaliana, cryptochrome1 (CRY1) and CRY2 primarily mediate blue light inhibition of hypocotyl elongation and photoperiodic promotion of floral initiation, respectively, although their functions do overlap. The cry1cry2 double mutant flowered later than wild-type under long day conditions. Through activation tagging, we identified an early flowering mutant called scc17-D (suppressor of cry1cry2#17-Dominant). TAIL-PCR results showed that a T-DNA insertion located between At1g25440 and At1g25450 in the first chromosome is responsible for the scc17-D mutant. The scc17-D mutant displayed dwarfism, reduction in leaf size, reduced length of siliques, and the altered shape of cotyledon pavement cells.关键词
拟南芥/隐花色素/光周期/激活标签/开花Key words
Arabidopsis thaliana/ cryptochrome/ photoperiod/ activation tagging/ flowering分类
生物科学引用本文复制引用
陈福禄,李宏宇,林辰涛,傅永福..拟南芥隐花色素突变体抑制子的筛选及其表型分析[J].中国农业科技导报,2009,11(3):93-97,5.基金项目
国家自然科学基金项目(30570162)资助. （30570162）
