安徽农业科学2009，Vol.37Issue(20)：9405-9406,2.
桫椤atpB-rbcL序列2种测序方法的比较分析
The Comparative Analysis of atpB-rbcL Sequence in Alsophila spinulosa Obtained by Direct and Cloned Sequencing
李媛 1侯可雷 1张静 2赵涛 3刘志兵3
作者信息
- 1. 日照职业技术学院,山东日照,276826
- 2. 山东省青岛市城市园林局浮山管理处,山东青岛,266200
- 3. 山东省徂徕山林场,山东泰安,271000
- 折叠
摘要
Abstract
[Objective]The research aimed to study the sequencing effects of atpB-rbcL sequence of Alsophila spinulosa with two different methods.[Method]The results of direct sequencing of PCR products or sequencing by cloning PCR products of atpB-rbcL uncoding region in A.spinuosa were compared and analyzed.[Result]The results showed that the profile of direct sequencing was disordered and overlapped after PolyT.The profile of cloned sequencing was clear and did not overlap.[Conclusion]Direct sequencing of PCR products was better than sequencing by cloning PCR products of the atpB-rbcL sequence in A.spinulosa.关键词
桫椤/PCR产物直接测序/PCR产物克隆测序/atpB-rbcL非编码区Key words
Alsophila spinulosa/Sequence analysis with PCR products/Sequence analysis with clone of PCR products/atpB-rbcL noncoding sequence分类
农业科技引用本文复制引用
李媛,侯可雷,张静,赵涛,刘志兵..桫椤atpB-rbcL序列2种测序方法的比较分析[J].安徽农业科学,2009,37(20):9405-9406,2.
