中国农业科学（英文版）2004，Vol.3Issue(12)：943-947,5.
The Protection Efficacity of DNA Vaccine Encoding Hemagglutinin of H5 Subtype Avian Influenza Virus
The Protection Efficacity of DNA Vaccine Encoding Hemagglutinin of H5 Subtype Avian Influenza Virus
JIANG Yong-ping 1YU Kang-zhen 1DENG Guo-hua 1TIAN Guo-bin 1QIAO Chuan-ling 1CHEN Hua-lan1
作者信息
- 1. Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R.China
- 折叠
摘要
Abstract
The DNA vaccine pCIHA5 encoding hemagglutinin can protect SPF chicken against lethal H5N1 avian influenza virus challenge. The more characters about its protection efficacity were studied. The protective rates in 10, 40, 70, 100 and 150μg groups immunized with pCIHA5 were 12.5 (1/8), 58.3 (7/12), 72.7 (8/11), 50.0 (6/12) and 66.7% (8/12), respectively. The protective rates in 5, 20, 35 and 50μg groups were 145.5 (5/11), 58.3 (7/12), 58.3 (7/12) and 91.7% (11/12), respectively. The 70, 100 and 5μg groups have virus shedding of 1/8, 2/6 and 1/5. Though the inactived oil-emulsion vaccine has high HI antibody titers and 100% protective rate, the AGP antibody could be detected after vaccination. Results show that the pCIHA5 is fit to boost by intramuscular injection. This would be useful to the study on gene engineering vaccine of avian influenza virus.关键词
Avian influenza virus/Hemagglutinin/DNA vaccine/Protection efficacityKey words
Avian influenza virus/Hemagglutinin/DNA vaccine/Protection efficacity引用本文复制引用
JIANG Yong-ping,YU Kang-zhen,DENG Guo-hua,TIAN Guo-bin,QIAO Chuan-ling,CHEN Hua-lan..The Protection Efficacity of DNA Vaccine Encoding Hemagglutinin of H5 Subtype Avian Influenza Virus[J].中国农业科学（英文版）,2004,3(12):943-947,5.
