南京航空航天大学学报（英文版）2002，Vol.19Issue(1)：94-97,4.
表面活性剂十二烷基磺酸钠(SDS)对核酸-Tb3+体系荧光性质的影响
EFFECT OF SURFACTANT SDS ON DETERMINATION OF NUCLEIC ACID WITH TERBIUM (Ⅲ) FLUORESENCE
吴翚 1宛瑜1
作者信息
- 1. 南京航空航天大学材料科学与技术学院,南京,210016,中国
- 折叠
摘要
Abstract
The effect of an anionic surfactant (sodium dodecyl sulfate, SDS) on the fluorescence properties of nucleic acid with terbium (III) is studied. Results show that ribonucleic acid (RNA) presents fluorescence reaction with Tb (III) directly, but deoxyribonucleic acid (DNA) presents similar fluorescence reaction only after its denaturation. In the presence of SDS, the fluorescence intensity is 4.0 times and 3.5 times greater than that of DNA and RNA without SDS.关键词
荧光性质/表面活性剂/DNA/RNA/铽Key words
fluorescence properties/surfactant/DNA/RNA/terbium分类
化学化工引用本文复制引用
吴翚,宛瑜..表面活性剂十二烷基磺酸钠(SDS)对核酸-Tb3+体系荧光性质的影响[J].南京航空航天大学学报（英文版）,2002,19(1):94-97,4.
