中国农业科学（英文版）2002，Vol.1Issue(9)：949-953,5.
Studies on the Primers Screening for AFLP Fingerprints of Rice Cultivars
Studies on the Primers Screening for AFLP Fingerprints of Rice Cultivars
ZHANG Chun-qing 1JIA Ji-zeng1
作者信息
- 1. Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing 100081 , P. R. China
- 折叠
摘要
Abstract
AFLP(amplified fragment length polymorphism) is a very powerful fingerprinting technology.The key of making variety fingerprints is to select specific powerful primers for each crop. A quick and effective procedure for selecting AFLP primers for rice variety fingerprinting was established as the following: (1)Choose 3 or more group materials that have close genetic relations. (2) Select potential polymorphic primers from primer pairs that are 2 + 2 primer crosses and same at two ends. (3) Recombine the selected potential polymorphic primers and choosing more polymorphic primers. (4) Add one selecting base at one end to become 2 + 3 or 3 + 2primers and further selecting more polymorphic primers. Some primers were selected with this procedure, such as M21Ps7 and M73P17, with which the fingerprints had more polymorphism and high quality.关键词
Rice/AFLP fingerprint/Primer selectionKey words
Rice/AFLP fingerprint/Primer selection引用本文复制引用
ZHANG Chun-qing,JIA Ji-zeng..Studies on the Primers Screening for AFLP Fingerprints of Rice Cultivars[J].中国农业科学（英文版）,2002,1(9):949-953,5.
