首页|期刊导航|中国神经再生研究（英文版）|Inhibitory effect of synthetic small interfering RNAs on glial fibrillary acidic expression in astrocytes
中国神经再生研究（英文版）2008，Vol.3Issue(9)：950-953,4.
Inhibitory effect of synthetic small interfering RNAs on glial fibrillary acidic expression in astrocytes
Inhibitory effect of synthetic small interfering RNAs on glial fibrillary acidic expression in astrocytes
摘要
Abstract
BACKGROUND: Glial fibrillary acidic protein (GFAP) expression highly correlates with spinal glial scar formation, and is regarded as an important target for scar therapy. Efficient inhibition of expression could benefit recovery from spinal cord injury. OBJECTIVE: To investigate the inhibitory effects of synthetic small interfering RNAs (siRNAs) on astrocytie GFAP expression in rats. DESIGN, TIME AND SETTING: A randomized, controlled, animal experiment at the cellular and molecular level was performed at the First Hospital of Dalian Medical University between June 2005 and February 2006. MATERIALS: A total of 100 seven-day-old, Sprague Dawley rats were selected. GAPDH siRNA was purchased from Ambion, USA, And TransMessengerTM Transfection Reagent from DAKO, Carpinteria, CA. METHODS: Rat astrocytes were isolated and cultured. Three pairs of 21-nucleotide (nt) siRNAs specific to rats GFAP mRNA, 401,404 and 854, were synthesized and transfected in primary astrocytes at 1, 2, 3, and 4 g/L using TransMessengerTM Transfection Reagent. Non-transfected astrocytes served as the blank group. Cells transfected with siRNA were regarded as the negative control group, with GAPDH siRNA as the positive control group, and 401 siRNA, 404 siRNA, and 854 siRNA as experimental groups. MAIN OUTCOME MEASURES: GFAP mRNA and protein expression were assessed by RT-PCR and Western blot, respectively, at 24, 48, and 72 hours of culture. RESULTS: GFAP mRNA expression in the positive control group was significantly less than the negative control group (P<0.01). GFAP mRNA expression in astrocytes from three pairs of siRNA was significantly less than the blank group after 48 hours (P<0.01 ), while no differences were detected between the negative control and blank groups (P>0.05). GFAP protein expression was remarkably less in siRNA-transfected astroeytes compared to the blank control (P < 0.01). CONCLUSION: Transfected siRNAs could significantly inhibit GFAP gene expression in astrocytes after 72 hours in culture.关键词
glial fibrillary acidic protein/RNA interference/spinal cord injuryKey words
glial fibrillary acidic protein/RNA interference/spinal cord injury分类
医药卫生引用本文复制引用
Mingzhu Zhang,Qing Zhao,Xin Tang,Guangrong Yu..Inhibitory effect of synthetic small interfering RNAs on glial fibrillary acidic expression in astrocytes[J].中国神经再生研究（英文版）,2008,3(9):950-953,4.基金项目
This study was supported by the Natural Science Foundation of Liaoning Province (No.20052165). （No.20052165）
