南京师大学报（自然科学版）2001，Vol.24Issue(1)：95-100,6.
FSH对人腔前卵泡体外生长的影响
The Effect of FSH on Human Preantral Follicles Cultured in Vitro
施旭东 1范必勤 2王公金 2曹步凯 2陈杰 1谭笑梅 3陈华 3孙贝加 4王金锋 5糜祖煌6
作者信息
- 1. 南京农业大学动物生理生化研究室,
- 2. 江苏省农科院牧医所胚胎工程实验室,
- 3. 南京市妇幼保健院,
- 4. 徐州市第四人民医院,
- 5. 徐州市妇幼保健院,
- 6. 无锡市克隆遗传技术研究所,
- 折叠
摘要
Abstract
To investigate the effect of FSH on the growth of human preantralfollicles cultured in vitro.FSH with different concentrations was added to the medium.The survival time and growth of follicles were observed and the levels of E2 was measured.The survival time of follicles cultured in vitro were 2.80±1.69 days for the control group ,5.36±0.63,5.47±2.50 and 8.13±4.19 days forⅠ,Ⅱand Ⅲ groups with FSH 0.5-IU/mL,1.0-IU/mL and 2.0-IU/mL respectively. Compared with control group,the statistics test results were P＜0.05,P＜0.01 and P＜0.001 respectively.The rates of follicular diameter increasing were 20.00% for the control group,35.71%,60.00% and 81.25% forⅠ,Ⅱand Ⅲ groups respectively.Compared with control group,the statistics test results were P＞0.05,P＜0.05,P＜0.01 respectively.The amount of E2 secreted by follicles were 49.94±20.91-pg/mL for control group, 108.63±41.06 ,315.53±93.39 ,438.24±187.61-pg/mL for Ⅰ,Ⅱ，Ⅲ groups respectively.The statistics test results were P＜0.001,P＜0.001,P＜0.05 between control and Ⅰ groups,Ⅰ and Ⅱ groups,Ⅱ and Ⅲ groups respectively.By adding FSH,the survival time of follicles cultured in vitro was lengthened and the follicles were growing larger.The secretion of E2 was increased.The relation between them was approximately positive correlative.关键词
人/FSH/腔前卵泡/体外培养/E2分类
生物科学引用本文复制引用
施旭东,范必勤,王公金,曹步凯,陈杰,谭笑梅,陈华,孙贝加,王金锋,糜祖煌..FSH对人腔前卵泡体外生长的影响[J].南京师大学报（自然科学版）,2001,24(1):95-100,6.
