首页|期刊导航|高技术通讯（英文版）|Screening and optimization of EPA-producing antarctic psychrophilic bacterium Shewanella sp.NJ136
高技术通讯（英文版）2007，Vol.13Issue(1)：95-102,8.
Screening and optimization of EPA-producing antarctic psychrophilic bacterium Shewanella sp.NJ136
Screening and optimization of EPA-producing antarctic psychrophilic bacterium Shewanella sp.NJ136
摘要
Abstract
Two hundred strains of bacteria from Antarctic sea ice were collected and screened for their ability of producing eicosapentaenoic acid(EPA, 20:5ω3)by means of Gas Chromatography/Mass Spetrometry (GC/MS). Eight strains of bacteria containing EPA were investigated, among which the outstanding one was recorded as NJl36. This bacterium was identified as Shewanella by the biochemical characteristics and 16S rRNA sequence. Response surface methodology(RSM)was applied to optimize the medium ingredients. A 24full factorial central composite design(FFCCD)was employed to determine the maximum EPA production at optimum levels of pH, NaCl, glucose and yeast extract. The predicted optimal combination of media constituents for maximum 14.02 mg/g(about 1.7-fold increase)EPA production were determined as 30.15‰(m/v)NaCl, 9.98g/L glucose, 4.42g/L yeast extract and pH 6.08. The actual experimental results were in agreement with the prediction.关键词
EPA/psychrophilic/Shewanella/antarctic/RSMKey words
EPA/psychrophilic/Shewanella/antarctic/RSM分类
生物科学引用本文复制引用
Zhang Botao,Miao Jinlai,Ma Jinhai,Zheng Zhou ,Wang Guodong,Wang Quanfu,Li Guangyou,Liu Wanshun..Screening and optimization of EPA-producing antarctic psychrophilic bacterium Shewanella sp.NJ136[J].高技术通讯（英文版）,2007,13(1):95-102,8.基金项目
Supported by the National Natural Science Foundation of China(No.40206022 & No.40506005). （No.40206022 & No.40506005）
