首页|期刊导航|中国神经再生研究（英文版）|Acupuncture at the San Jiao meridian affects brain stem issue G protein content in a rat migraine model
中国神经再生研究（英文版）2008，Vol.3Issue(9)：958-961,4.
Acupuncture at the San Jiao meridian affects brain stem issue G protein content in a rat migraine model
Acupuncture at the San Jiao meridian affects brain stem issue G protein content in a rat migraine model
摘要
Abstract
BACKGROUND: G protein is closely associated with vasomotion. Vasomotor dysfunction accompanies migraine attack. OBJECTIVE: To investigate the effects of the San Jiao meridian acupuncture on G protein content in a rat migraine model. DESIGN, TIME AND SETTING: The present randomized grouping, cellular and molecular biological level trial was performed at the Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University & Key Laboratory for Tumor Proteomics of Ministry of Health between October 2003 and June 2004. MATERIALS: Forty healthy, male, Sprague Dawley rats were included in this study. The G6805-2A elector-acupuncture apparatus was a product of Shanghai Huayi Medical Instrument Factory, China. Nitroglycerin was produced by Guangzhou Mingxing Pharmaceutical Factory, China. Antibodies against inhibitory and stimulatory G proteins were purchased from Sigma Chemical Company, USA. METHODS: All 40 rats were randomly and evenly divided into 4 groups. In the blank control group, the rats remained untouched. Rats from the normal control group were subcutaneously administered 2 mL/kg physiological saline. In the model group, migraine was induced with a subcutaneous injection of 10 mg/kg nitroglycerin (5 g/L), and the rats received no further treatment. In the acupuncture-treated group, 30 minutes after migraine induction, acupuncture was performed at the bilateral Waiguan (SJ 5) and Yifeng (SJ 17) points, with an acupuncture depth of 1 mm. Electric-stimulation parameters of 20 Hz for low frequency, 40 Hz for high frequency, and 0.5-1.0 mA for current intensity were set. Ten acupuncture sessions were applied, with 20-minute low-frequency and 20-minute high-frequency stimulation and 3 seconds of interval time. MAIN OUTCOME MEASURES: Inhibitory and stimulatory G protein contents were detected by Western blot analysis. RESULTS: At 4 hours after migraine induction, compared with the blank control and normal control groups, stimulatory G protein concentration was significantly increased, while inhibitory G protein levels were significantly decreased in the model group (P < 0.01). In the acupuncture-treated group, both stimulatory and inhibitory G protein concentrations were significantly increased following acupuncture treatment (P < 0.01 ), but stimulatory G protein levels were less and the inhibitory G protein concentrations were greater compared to the model group (P < 0.01). There was no significant difference in stimulatory and inhibitory G protein levels between the blank control and normal control groups (P > 0.05). CONCLUSION: Dysfunctional G protein signal transductions in the rat brain stem may be responsible for migraine attack. Acupuncture at the San Jiao meridian ameliorates migraines by mediating the G protein signal transduction pathway.关键词
acupuncture/G protein/migraine/San Jiao meridian/Western blotKey words
acupuncture/G protein/migraine/San Jiao meridian/Western blot分类
医药卫生引用本文复制引用
Sue Wang,Wei Li,Guangwei Zhong,Zhenyan Li,Lingbo Wen..Acupuncture at the San Jiao meridian affects brain stem issue G protein content in a rat migraine model[J].中国神经再生研究（英文版）,2008,3(9):958-961,4.基金项目
This study was supported the Hunan Provincial Administration of Traditional Chinese Medicine, No.200125.Hunan Provincial Administration of Traditional Chinese Medicine, No.200125.This project has been awarded second prize of Hunan Provincial Science and Technology Advancement in Traditional Chinese Medicine, No. 2005012. （）
