中华医学杂志（英文版）2002，Vol.115Issue(7)：963-967,5.
内毒素诱导的急性肺损伤大鼠呼吸膜AQP1及AQP5表达的研究
Decreased expression of AQP1 and AQP5 in acute injured lungs in rats
摘要
Abstract
Objective To determine if aquaporin1 (AQP1) and aquaporin5 (AQP5) are expressed in the alveolar capillary membrane in rats. Moreover, to investigate the alteration of AQP1 and AQP5 in acute injured lungs.Methods The distribution of AQP1 and AQP5 in alveolar capillary membrane were investigated by immunohistochemistry and immunoelectron microscopy with affinity-purified antibodies to human AQP1 and AQP5. To study the possibility that alveolar capillary membrane AQP1 and AQP5 undergo altered regulation, we established a rat model using alveolar instillation of lipopolysaccharide (LPS). Results Immunolabelling showed AQP1 was stained primarily in the microvascular endothelia of normal lungs, while AQP5 was expressed in type Ⅰ pneumocytes. Immunohistochemical analysis showed a significant decrease in the expression of AQP1 and AQP5 in injured lungs at 4h-48h after LPS instillation. AQP1 protein was resumed partly at 24h after LPS instillation and steroid administration, whereas AQP5 was unchanged. Conclusion The decreased expressions of AQP1 and AQP5 in injured lungs suggest that both of them may play a role in abnormal fluid transportation.关键词
AQP1 AQP5 急性肺损伤 激素Key words
aquaporin 1/aquaporin 5/acute lung injury/steroid分类
医药卫生引用本文复制引用
焦光宇,李尔然,于润江..内毒素诱导的急性肺损伤大鼠呼吸膜AQP1及AQP5表达的研究[J].中华医学杂志（英文版）,2002,115(7):963-967,5.基金项目
This project was supported by the National Natural Science Foundation of China (No. 39870338). （No. 39870338）
