中国临床康复2004，Vol.8Issue(5)：966-967,2.
碱性成纤维细胞生长因子对大鼠脊髓损伤后神经细胞凋亡的抑制
Inhibition effect of basic fibroblast growth factor on apoptosis following spinal cord injury in rats
摘要
Abstract
AIM: To explore the inhibition effect of basic fibroblast growth factor(bFGF) on apoptosis following spinal cord injury in rats.METHODS: SD rats were suffered (10 g ×2.5 cm)impaction to prepare a T8 spinal cord injury model on following the technique of Allen Weight drop (WD) method. A thin plastic tube was gently inserted into the subarachnoid space below the injury level. In the bFGF group(group A), 20 μL bFGF (bFGF 100 u)was injected shortly after the injury and 1,2,4,8,12,24 and 48 hours post the operation via the tube; bFGF was injected once per week at dosage of 20 μL; The saline group(group B): 30 spinal cord injury animal models were prepared, the same volume of normal saline was given to replace bFGF at the same period as control. TUNEL technique was used to determine apoptosis cells at 1, 3, 7, 14, 28 days after the injury. Apoptosis index(AI, AI = Number of apoptosis cell' s nucleus/Number of total cells' nucleus)was calculated by a computer image quantitative analysis system.RESULTS: Apoptosis cells were observed in both of the two groups. AI at different time period was (4.57±0.43), (5.38 ±1.16), (3.43±0.65), (3.38±0.58), (2.63±0.43) in group A; and (7.36 ±0.68), (13.96±2.74), (9.26±1.03), (7.25±0.73), (5.79±0.57)ingroupB. AI of group B was apparently higher than that of group A.CONCLUSION: bFGF can inhibit cell apoptosis in the injured area of spinal cord.关键词
脊髓损伤/脱噬作用/bFGF分类
医药卫生引用本文复制引用
刘文革,林佳俊,王锋,陈敏..碱性成纤维细胞生长因子对大鼠脊髓损伤后神经细胞凋亡的抑制[J].中国临床康复,2004,8(5):966-967,2.基金项目
福建省自然科学基金(F 0110004) （F 0110004）
福建省卫生厅青年基金(2001-1-10)资助 （2001-1-10）
