中国病理生理杂志2001，Vol.17Issue(2)：97-100,4.
稳定表达鼻咽癌来源潜伏膜蛋白1基因的鼻咽癌细胞系的建立
Establishment of nasopharyngeal carcinoma (NPC) cell lines stable expressing NPC-derived LMP1
摘要
Abstract
AIM: To establish nasopharyngeal carcinoma(NPC) cell lines stableexpressing NPC-derived latent membrane protein 1(LMP1) gene. METHODS: General expression vector and epithelium-specific expression vector of NPC-derived LMP1 gene were constructed by using recombinant techniques, then transfected these vectors into a poor differentiated NPC cell line named CNE-2 ,integration and expression of N-LMP1 in CNE-2 cells were detected by PCR,RT-PCR and Western blot. RESULTS: (1) General expression vector and epithelium-specific expression vector of NPC-derived LMP1 gene were constructed successfully.(2) It showed that N-LMP1 gene expressed in CNE-2 cells correctly. CONCLUSION: The first NPC cell lines which stable express NPC-LMP1 were established. The cell lines obtained will provide important basis for exploring the role of NPC-LMP1 in nasopharynx carcinogenesis.关键词
膜蛋白类/爱泼斯坦-巴尔病毒/鼻咽肿瘤分类
生物科学引用本文复制引用
蓝轲,甘润良,任彩萍,何志巍,谢鹭,姚开泰..稳定表达鼻咽癌来源潜伏膜蛋白1基因的鼻咽癌细胞系的建立[J].中国病理生理杂志,2001,17(2):97-100,4.基金项目
国家自然科学基金重点项目 （）
美国中华医学基金资助项目 （）
