山西医科大学学报2007，Vol.38Issue(2)：97-102,6.
早期惊厥样放电对发育中神经元NMDA受体1表达的远期影响
Long-term effect of early discharges on NMDAR-1 expression in developing neurons
摘要
Abstract
Objective To explore the long-term effect of early discharges on NMDAR-1 expression in developing rat cortical neurons. Methods The seizure model was recurrent epileptiform discharges induced by transient magnesium-free media treatment in the primary cultured embryo cortical neurons of rat. According to different treatment to cortical neurons at cultured day 6, cells were assigned to three groups respectively based on the mediums they were transiently exposed to. The neurons were exposed to Dulbecco's Modified Eagle's Medium (DMEM) in control group 1 (CONT1), exposed to a physiological solution (PS) in control group 2(CONT2) ,and exposed to the same medium as CONT2 except for the removal of magnesium in the magnesium-free physiological solution (MGF) group. Following a 3 h exposure, the expression of N-methyl-D-aspartate receptor 1 (NR1) mRNA and protein were measured at different culture days (7,12,17) using real-time PCR and Western blot assays. Results The expression of NR1 mRNA and protein in DMEM medium had higher value level at culture day in vitro (DIV) 7,and then they decreased gradually and had the lowest value at DIV12. They increased again with development. Compared with groups CONT1 and CONT2 respectively,expression of NR1 mRNA and protein in group MGF decreased at DIV7 and increased at DIV12 (P＜0.05) ,and there were no statistic significant differences at DIV17. Conclusion The results suggest that NR1 mRNA and protein may alter with the development and there are long-term effects of Mg2+-free induced seizure-like discharges on expression of NR1 mRNA and protein in developing rat cortical neurons.关键词
发作/受体,N-甲基-D-天冬氨酸/大鼠Key words
seizures/receptors,N-methyl-D-aspartate/rats分类
医药卫生引用本文复制引用
王静敏,姜玉武,姜茜,曹海燕,倪宏,薄涛,吴希如..早期惊厥样放电对发育中神经元NMDA受体1表达的远期影响[J].山西医科大学学报,2007,38(2):97-102,6.基金项目
国家自然科学基金资助项目(30270448,30470555) （30270448,30470555）
北京大学国家"211工程"资助项目 （）
第三十三届博士后科学基金资助项目(2003033082) （2003033082）
