大豆科学2000，Vol.19Issue(2)：97-104,8.
利用RAPD技术分析野生大豆×栽培大豆后代品系遗传组成
RAPD ANALYSIS OF THREE SOYBEAN LINES FROMTHE CROSS OF ( Glycine soja × Glycine max) × G. max
摘要
Abstract
Three soybean elite lines(8650-1-4 and 8702-2-4, 8702-2-8) wasdeveloped by using Glycine soja as female parent and Glycine maa as male parent. 8650-1-4 was developed by backcrossing of Tongjiao 81-1543 with F1 of ( G. soja × Chengdou No. 1), and 8702-2-4, 8702-2-8 were developed by backcrossing of Tongjiao 81-1543 with F2 of ( G. soja × Chengdou No. 1). Parental and progeny nuclear DNA was analysised by RAPD method. A total of 79 primers were analyzed with parents, fifteen of which were polymorphic between parents. Using these 15 primers, G. Soja amplified 19 special loci, 4 in Chengdou and 9 in Tongjiao respectively, which indicates that genetic background of G. soja differ from G. max. The number of special loci coming from G. soja , Chengdou No. 1 and Tongjiao 81-1543 is 2,2 and 5 for 8702-2-4;. 1,2 and 8 for 8702-2-8; 11,1 and 2 for 8650-1- 4, respectively. These results were in accordance with their agronomic character performance, 8702-2-8 was more similar to Tongjiao 81-1543 and 8650- 1-4 inherited more characters from G. soja , which indicated that RAPD markers is an useful tool to study relationship between parents and progenies . In addition , we also found some RAPD loci were stronger in 8702-2-4 and 8702-2-8 than their parents, but the mechanism is not clear.关键词
大豆品系/野生大豆/栽培大豆/RAPDRAPD ANALYSIS OF THREE SOYBEAN LINES FROMTHE CROSS OF ( Glycine soja × Glycine max) × G. maxZheng Cuiming（Institute of Crop Germplasrn Resources,CAAS,Beijing 100081,China） Chang Ruzhen（Institute of Crop GermplasrnKey words
Soybean lines/Glycine soja × Glycine mar/RAPD markers分类
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郑翠明,李玉清..利用RAPD技术分析野生大豆×栽培大豆后代品系遗传组成[J].大豆科学,2000,19(2):97-104,8.基金项目
河北省自然科学基金 （）
