中国临床康复2004，Vol.8Issue(5)：972-973,2.
内皮差异表达基因消减文库的构建及在创伤修复中的应用
Construction of the subducting cDNA library of differentially expressed genes in endothelium and its application in trauma repairs
摘要
Abstract
AIM: To construct a subducting cDNA library of differentially expressed genes after the stimulation of endotoxin to human umbilical vein endothelial cells(HUVECs). METHODS: mRNAs of cultured HUVECs stimulated by endotoxin after 6h were extracted, and then cDNA was synthesized by reverse transcription. Suppressive subducting hybridization was used to enrich the differential expression of genes. T/A plasmid was subcloned and competence escherichia coli was conversed.RESULTS: Two subducting cDNA iibraries of up-reguiation and down-regulation differentially expressed genes in HUVECs after stimulated by endotoxin were constructed successfully. It was the basis for screening new endothelial cells to activate the related genes. CONCLUSION: The subducting cDNA hbrary constructed by suppressive subducting hybridization enriched and homogenized the differentially expressed genes of different degrees of abundance stimulated by endotoxin. It is important for understanding the activation mechanism of endothelial cells and their role in the process of tissue repair.关键词
内皮,血管/细胞/内毒素类/DNA,互补/遗传学分类
医药卫生引用本文复制引用
梁自文,罗向东,杨宗城..内皮差异表达基因消减文库的构建及在创伤修复中的应用[J].中国临床康复,2004,8(5):972-973,2.基金项目
国家自然基金青年基金(30200101) （30200101）
