山西医科大学学报2001，Vol.32Issue(2)：97-99,3.
人肝细胞生长因子β链基因的克隆及表达载体的构建
The clone of human hepatacyte growth factor β chain gene and the construction of its expressing system
解军 1牛勃 1程牛亮 1吴玲 1王惠珍 1杨涛 1常冰梅1
作者信息
摘要
Abstract
Objective To construct a recombinant human hepatocyte growth factor expressing system as to study in the field of expressing regulation and biological, medical function of HGF. Methods Total RNA was extracted from human fetal liver as a sample. Using the technics of RT-PCR, the clone of recombinant human hepatocyte growth factor was constructed by processing and modifying HGF gene in pre-transcription period time and inserting into a vector, pBV220. Then, the recombinant clone was analysed by restriction enzyme maps. The insen gene of clone was sequenced by auto-sequencing instrument. Results The integrity cDNA sequence of HGF-β was obtained by construction system. The result of restriction enzyme map analysis showed the same site as the plan designed before experiment. Conclusion It is suggested that the clone of human hepatocyte growth factor β chain gene is succeeded to construct in the end.关键词
肝细胞生长因子/分子克隆/DNA,重组/基因表达分类
生物科学引用本文复制引用
解军,牛勃,程牛亮,吴玲,王惠珍,杨涛,常冰梅..人肝细胞生长因子β链基因的克隆及表达载体的构建[J].山西医科大学学报,2001,32(2):97-99,3.
