中国农业科技导报2009，Vol.11Issue(3)：98-101,4.
过量表达紫茎泽兰类黄酮3'-羟化酶基因对转基因烟草POD、PAL活性的影响
Effects of Eupatorium adenophorum Flavonoid 3'-Hydroxylase Over-expression on POD and PAL Activity in Transgenic Tobacco
摘要
Abstract
Eupatorium adenophorum has strong allelopathy, and can suppress all plants around it. Flavonoid 3'-hydroxylase belongs to cytochrome P450(CYP) monooxygenase, which can catalyze oxidation reactions that depend on the substrate NADPH or NADH, and play an important role in plant allelopathy. Using F3'H gene transgenic tobacco as material, POD and PAL activities of transgenic tobacco were tested compared with that in control tobacco and transgenic tobacco with pBI121 vector. The results showed that POD activity value of transgenic tobacco with F3'H gene is 3.8 times to control tobacco, twice to transgenic tobacco transformed pBI121 vector, while PAL activity value of transgenic tobacco with F3'H gene is 4 times to control tobacco, twice to transgenic tobacco transformed pBI121 vector. So we speculate that F3'H gene is considered important for defense against exogenous damage.关键词
类黄酮3'-羟化酶/过表达/POD/PALKey words
flavonoid 3'-hydroxylase/ over-expression/ POD/ PAL分类
生物科学引用本文复制引用
张松焕,李春奇,郭惠明,裴熙祥,程红梅..过量表达紫茎泽兰类黄酮3'-羟化酶基因对转基因烟草POD、PAL活性的影响[J].中国农业科技导报,2009,11(3):98-101,4.基金项目
国家自然科学基金项目(30771422) （30771422）
国家973计划项目(2009CB119201)资助. （2009CB119201）
