南京师大学报（自然科学版）2001，Vol.24Issue(4)：98-106,9.
水溶液中的环状DNA超螺旋空间构象
Superhelical Spatial Conformation on Circular DNA in Aqueous Solution
余多慰 1柯惟中 2吴宇澄 3谭锡林3
作者信息
- 1. 南京师范大学生命科学学院，南京210097
- 2. 江苏省光电技术重点开放实验室，南京210097
- 折叠
摘要
Abstract
The circular DNA has been shown a regular superhelical spatial conformation.The Raman spectra of bacterial plasmid DNA in aqueous solution were recorded.Both diagnostic bands at the 854 and 1-083-cm-1 reflecting the vibration state of the tertiary structure of the DNA main backbone are usually hard to find in the Raman spectra of the linear DNA molecule and their assignments have been discussed.The intensity of the peak at 969-cm-1,corresponding to the C'-C' stretching vibration of the deoxyribose,is between that of calf thymus DNA in aqueous solution and in fiber (0.46＜0.83＜1.10).As a result of analyzing base stacking,the existence of the superhelical spatial conformation represents both the increases in the staking reaction activity of thymine residues in the DNA molecule and the damage of a considerable amount of the Watson-Crick type hydrogen-bonding in the AT base pairs.Meanwhile,the characteristic band at 1-378-cm-1,corresponding to C-N and C=O group vibration,indicating the composition of H-bond of the thymine,has markedly Raman hypochromism.It is a sufficient basis of confirming the tertiary structure of circular DNA molecule with Raman characteristic bands.关键词
质粒DNA/三级结构/喇曼光谱/超螺旋分类
化学化工引用本文复制引用
余多慰,柯惟中,吴宇澄,谭锡林..水溶液中的环状DNA超螺旋空间构象[J].南京师大学报（自然科学版）,2001,24(4):98-106,9.
