中国临床康复2004，Vol.8Issue(5)：982-983,2.
保肢手术的前瞻性基因疗法研究:BLCAP基因在人骨肉瘤细胞发展过程中的调节作用
A prospective genetic therapy study of extremity saving operations:the regulatory function of gene BLCAP in the development of human osteosarcoma
摘要
Abstract
AIM: To explore the effect of BLCAP antisense oligonucleotide on human osteosarcoma cell line SOSP-9607 to provide a prospective research rationale for extremity saving operations. METHODS: Using BLCAP antisense oligonucleotide on cultured human osteosarcoma cell line SOSP-9607. The cell growth curve was depicted, cell colony forming rate was calculated, and cell cycle was measured. RESULTS: Cell growth and colony forming ability were apparently stimulated when the content of BLCAP antisense oligonucleotide was 10 μ mol/L.Also, flow cytometry indicated that the cell cycle changed and it promoted cell proliferation activity. CONCLUSION: The present study found for the first time that BLCAP antisense oligonucleotide had an obvious effect on human osteosarcoma cell line SOSP-9607, suggesting that the gene BLCAP may function as a anti-oncogene in the development of human osteosarcoma.关键词
基因/寡核苷酸类,反义/骨肉瘤/细胞周期分类
医药卫生引用本文复制引用
范德刚,范清宇,张惠中,张盈华,刘云燕..保肢手术的前瞻性基因疗法研究:BLCAP基因在人骨肉瘤细胞发展过程中的调节作用[J].中国临床康复,2004,8(5):982-983,2.基金项目
2001年第四军医大学博士学位论文课题资助项目(2001010) （2001010）
