中国临床康复2004，Vol.8Issue(5)：984-985,2.
骨巨细胞瘤组织尿激酶型纤溶酶原激活剂系统mRNA的表达与其生物学行为及复发的关系
Relationship between expression of urokinase-type plasminogen activator mRNA with giant cell tumor of bone and its pathological grading and recurrence
摘要
Abstract
AIM: To investigate the relationship between the expression of uroki- nase-type plasminogen activator(u-PA) mRNA in giant cell tumors of bone(GCT) and pathological grading and recurrence of GCT.METHODS: The expressions of u-PA, u-PA receptor(u-PAR) and u-PA inhibitors(PAI) -2 mRNA in the specimens of GCT obtained by surgical curettement from 42 cases, 13 of grade Ⅰ, 23 of grade Ⅱ, and 6 of grade Ⅲ,and 12 relapsing 5 -32 months later, were detected with in situ hybridization (ISH).RESULTS: ① The expression rates of u-PA, u-PAR, and PAI-2 mRNA in the multinuclear giant cells(MGCs) in the GCT tissues were 64.3%,71.4% and 40.5% respectively, and the expression rates in the mononuclear cells(MCs) were 54.8% , 45.2% and 9.5% respectively. ② The expression rate of u-PA mRNA in MGC, and the expression rates of u-PA and u-PAR mRNAs in MC were significantly higher in the cases of grade Ⅱ and Ⅲ than in the cases of grade I (X2 = 8.00, 7.82, and 6.81, all P ＜0. 05) and significantly higher in the non-recurrent group (X2 = 5.49, 5.54,and 9.84, P＜0.05). ③ A closely positive relationship was shown between the expression of u-PA mRNA in MGC and in MC ( P ＜ 0. 05 ), between the expression of u-PAR and of PAI-2 mRNA in MGC (x2 = 7.20, P ＜ 0. 05),and between the expression of u-PA and expression of u-PAR mRNA in MC (X2 =8.19, P ＜0.05) . The expression rate of PAI-2 mRNA in MGC was higher than that in MC significantly (x2 = 10. 73, P ＜ 0. 05).CONCLUSION: The expressions of u-PA and u-PAR mRNA in GCT tissues, especially in MC, are closely related to the malignancy of GCT.关键词
骨肿瘤/纤溶酶原激活剂/原位杂交分类
医药卫生引用本文复制引用
李海刚,董书堃,曾韵洁,沈溪明,曾弘,任明能..骨巨细胞瘤组织尿激酶型纤溶酶原激活剂系统mRNA的表达与其生物学行为及复发的关系[J].中国临床康复,2004,8(5):984-985,2.基金项目
广东省自然科学基金资助(970073) （970073）
