分析化学2009，Vol.37Issue(7)：985-988,4.
高灵敏度化学发光磁酶免疫法检测人绒毛膜促性腺激素
Detection of Human Chorionic Gonadotropin by Highly Sensitive Magnetic EnzymE-linked Chemiluminescent Immunoassay
摘要
Abstract
A highly sensitive magnetic enzymE-linked chemiluminescent immunoassay method was developed for the detection of human chorionic gonadotropin(HCG). The monoclonal antibody was covalently coupled on the surface of carboxylated magnetic beads to generate magnetic-biotargeting; Alkaline phosphatase(ALP) was utilized as a labeled reagent of another monoclonal antibody, whereas 3-(2-spimadamantane) 4-methoxy-4-(3-phosphoryloxy)phenyl-1,2-dioxetane(AMPPD) was utilized as the chemiluminescent substrate. Based on this concept, a highly sensitive chemiluminescent immunoassay method was established to test HCG. Then, several modifications were made to optimize the method, and the detection sensitivity and procedure were improved accordingly. The detection of the assay could be fulfilled within 60 min and the test result of HCG concentration was linear over the range of 0.15 150 IU/L with good relativity(r=0.960). The relative standard deviation(RSD) were below 5% and the sensitivity of this method was 0.15 IU/L. The proposed method with wide linear range, simple operation and fast detection showed good prospect in practical application on-site.关键词
化学发光/磁性微球免疫分析/人绒毛膜促性腺激素Key words
Chemiluminescence/magnetic immunoassay/human chorionic gonadotropin分类
化学化工引用本文复制引用
刘军涛,刘儒平,王蜜霞,刘春秀,罗金平,蔡新霞..高灵敏度化学发光磁酶免疫法检测人绒毛膜促性腺激素[J].分析化学,2009,37(7):985-988,4.基金项目
本文由国家高技术研究发展计划项目(Nos.2007AA03Z428、2007AA042105)、国家自然科学基金项目(No.60801032)、中科院创新项目(No.KGCX2-YW-111-2)、中科院电子所创新项目(No.08SQCX-9151)和中科院电子学青年创新项目(No.07QNCX-9240)资助 （Nos.2007AA03Z428、2007AA042105）
