分析化学2009，Vol.37Issue(7)：989-993,5.
高效液相色谱研究丝素亲核基团与一氯均三嗪型活性染料反应动力学
Reaction Kinetics of Monochlorotriazine Reactive Dyes with Neucleophilic Groups in Silk by High Performance Liquid Chromatography
摘要
Abstract
Using Isopropyl amine (0.003 mol/L), p-methyl-phenol (0.03 mol/L) and methanol (1 mol/L) for simulating amino groups, phenol groups and hydroxyl groups in silk fibroin respectively, the reaction rate of different nucleophilic groups in silk with monochlorotriazine reactive dye was studied at various temperature and pH by high performance liquid chromatography. It was found that the overall reaction rate of dyes′ phenolysis was by far higher than that of ammonolysis and alcoholysis on the conditions of 70-95 ℃ and pH=8-10. The phenolysis overall reaction rates of C.I. reactive red 24 and C.I. reactive orange 2 were 8.5 and 12.5 times of their ammonolysis′, and 23 and 50 times of their alcoholysis′ in the term of pH 9 and 95 ℃. The phenolysis efficiencies of those two dyes were 47.4 and 96.3, the ammonolysis efficiencies were 4.6 and 6.9, and the isopropyl amine, p-methyl-phenol and methanol to simulate the silk fibroin, the reaction selectivity between the monochlorotriazine reactive dyes and nucleophilic groups in silk fibroin was investigated. It was deduced that monochlorotriazine reactive dyes are suitable for dyeing silk on the conditions of about 85 ℃ and pH=8-9, and under this condition, phenol groups are the major groups in silk dyeing with monochlorotriazine reactive dyes, amino groups are the minor groups, and the contributions of hydroxyl groups can be neglected.关键词
反应动力学/一氯均三嗪型活性染料/蚕丝/亲核基团/高效液相色谱Key words
Reaction kinetics/monochlorotriazine reactive dye/silk fibroin/nucleophilic groups/high-performance liquid chromatography分类
化学化工引用本文复制引用
邵敏,邵建中,刘今强,李萍..高效液相色谱研究丝素亲核基团与一氯均三嗪型活性染料反应动力学[J].分析化学,2009,37(7):989-993,5.基金项目
本文系国家自然科学基金(No.20075023),浙江省自然科学基金重点项目(No.ZC0207)和浙江省科技计划项目(No.2208F70040)资助 （No.20075023）
