安徽农业科学2009，Vol.37Issue(21)：9902-9904,3.
家鸡胚胎发育过程DNA甲基化的MSAP检测
Detection of MSAP (Methylation Sensitive Amplified Polymorphism) on DNA Methylation during Chicken Fetation
班谦 1赵宗胜 1曹体婷1
作者信息
- 1. 新疆石河子大学动物科技学院,新疆石河子,832000
- 折叠
摘要
Abstract
[Objective]The study aimed to detect the DNA methylation level in different chicken fetation stages. [Method]The chicken embryo in different development stages was taken as the tested materials to extract the genomic DNA and its DNA methylation level was detected preliminarily by the technique of methylation sensitive amplified polymorphism (MSAP). [Result]It was found that the number of methylation sites were 880, in which, the single-stranded outboard cytosine methylation (hmCCGG) sites of CCGG sites accounted for 20.48%, the double-stranded inside cytosine methylation (Cm CGG) sites of CCGG sites accounted for 19.30%, resp., and the total of them was 39.78%. [Conclusion]The level of DNA methylation showed a rising trend along with the chicken fetation.关键词
甲基敏感扩增多态性/表观遗传/鸡胚/检测Key words
Methylation sensitive amplified polymorphism (MSAP)/Epigenetic inheritance/Embryo/Detection分类
农业科技引用本文复制引用
班谦,赵宗胜,曹体婷..家鸡胚胎发育过程DNA甲基化的MSAP检测[J].安徽农业科学,2009,37(21):9902-9904,3.
