摘要
Abstract
Objective To study the specificity of molecules CD1a and CD83 expression on the cultured dendritic cells in the peripheral blood of breast cancer patients.Methods Mononuclear cells of peripheral blood were isolated by Ficoll Hypaque centrifugation from 32 breast cancer patients and plated on 6-well culture plates(106 /ml, 2 ml/well) in RPMI1640 medium supplemented with 10% heat-inactivated fetal bovine serum, GM-CSF, IL-4, and/or TNF-α. Two h later, nonadherent cells were gently removed and fresh medium was added. Cultured cells were analyzed by flow cytometry with fluorescence labeled monoclonal antibodies (mAbs). The monoclonal antibodies used in this study included fluorescein isothiocyanate (FITC)-conjugated mAbs specific for CD1a, CD3, CD19, CD40, and CD80; phycoerythrin (PE)-conjugated mAbs specific for CD3, CD19, CD83, CD86, and MHC-Ⅱ. Cultured and fluorescence stained cells were taken pictures by confocal scanning microscope respectively.Results Cells displayed a characteristic phenotype such as CD1a+, CD40+, CD80+, CD86+ and CD83+. CD1a and CD83 molecules could also be expressed on the surface of CD3+ T lymphocytes and CD19+ B lymphocytes. The expression levels of CD1a and CD83 in activated lymphocytes were higher than those in the unactivated lymphocytes, and higher in B lymphocytes than in T lymphocytes.Conclusion The molecules of CD1a and CD83 are not specific phenotypes for dendritic cells. We now still need to apply both cell morphology and molecules such as CD1a, CD40, CD80, CD83 and CD86 to identify dendritic cells.关键词
树突状细胞/外周血/乳腺癌/CD1a/CD83Key words
dendritic cells/peripheral blood/breast cancer/CD1a/CD83分类
医药卫生
