波谱学杂志2001，Vol.18Issue(2)：99-104,6.
一种脑代谢研究的有效方法
——高分辨率磁共振波谱分析
A POWERFUL METHOD OF STUDYING METABOLISM
IN BRAIN——High Resolution Nuclear Magnetic
Resonance Spectroscopy
王克强 1王利民 1夏薇 2余亦华 2顾兴华 1贾剑国 1葛均波1
作者信息
- 1. 复旦大学附属中山医院心血管病研究所基础医学院解剖教研室，
- 2. 中国科学院上海有机化学研究所,
- 折叠
摘要
Abstract
In this paper, a howerful method was introduced to study the metabolism in brain. Adult rats were frozen with liquid-N\-2 through a self-command funnel. After perchloric acid (PCA) extracts were prepared and lyophilized, the residual substance were dissolved in D\-2O and investigated with 1H and 31P nuclear magnetic resonance spectroscopy (MRS). Result:High-resolution nuclear magnetic resonance spectroscopy of brain tissue was achieved. 31P MRS reveals ATP,ADP,phosphocreatine, inorganic orthophosphate and manifold aliphatic phosphates and sugar phosphates; 1H MRS shows many resonance peaks, including lactate, N-acetylaspartate, choline, creatine, GABA, myo-inositol, succinate and manifold amino acids. The peaks were integrated and the relative values might be used to express the metabolism or damnification of brain.关键词
脑/代谢/1H磁共振波谱/31P磁共振波谱分类
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王克强,王利民,夏薇,余亦华,顾兴华,贾剑国,葛均波..一种脑代谢研究的有效方法
——高分辨率磁共振波谱分析[J].波谱学杂志,2001,18(2):99-104,6.
