中国现代医学杂志2008，Vol.18Issue(8)：993-999,1004,8.
氧化苦参碱对CD4+CD25+调节性T细胞(Tr)和淋巴细胞增殖的影响
Effect of Oxymatrine on CD4+CD25+ regulatory T cells and lymphocyte proliferation
李建国 1伍斌 2谢红付 2张江林 2易梅 2李吉2
作者信息
- 1. 中南大学生物医学工程学院,湖南,长沙,410008
- 2. 中南大学湘雅医院,皮肤科,湖南,长沙,410008
- 折叠
摘要
Abstract
[Objective]To analyse Oxymatrine's(OMT)effects on the quantity of mouse's regulatory T cells(Tr cells)in the peripheral blood and mouse lymphocyte proliferation stimulated by con A,probe into the immunological mechanism that OMT treats allergic contact dermatitis(ACD).[Methods]Building up an ACD mouse medel stimulated by Dinitrofluorobenzene(DNFB),taking intraperitoneal injection (IP)by different dosage8 of OMT,PBS and Hydrocortisone(HCT),collecting blood In 1 d,7 d,14d,21 d and 28 d via mouse tail vein,then marking the T cells with anti-CD3,anti-CD4,anti-CD25 three-colored immune fluorescence antibody,using flow cytometer to detect the quantity the of CD4+ CD25+ T cells.To utilize Carboxyfluorescein diacetate succinimidyl ester(CFDA-SE)dyed and flow cytometer to examine the fluorescence intensity changes of lymphocytes co-stimulated by polyclonal stimulator Con A and OMT.[Results]500,125 and 31 μ.g/mL OMT has the ability to restrain the proliferation of lymphocyte of mouse depending on its concentration in substrate,but 16,8,4,2μgs/mL OMT improve the proliferation of T lymphocytes of mouse's lymph node,no obvious dependence on its concentration in substrate.Intraperitoneal injection of OMT can improve mouse CD4+ CD36+T cell quantities in peripheral blood obviously,compared OMT groups with HCT and PBS group(P<0.01).[Conclusions]The effects of OMT on the proliferation of lymDhocytes of mouse's lymph node T cells presents the two-way function,OMT can obviously improve the quantity of CD4+ CD25+T cells in the peripheral blood of mouse throush intraperitoneal injection,implying that OMT is a kind of two-way immunoregulator.关键词
氧化苦参碱/T淋巴细胞/CD4+CD25+T细胞Key words
Dinitrofluorobenzene(DNFB)/allergic contact dermatitis(ACD)/Oxymatrine(OMT)/resulatory T cell分类
医药卫生引用本文复制引用
李建国,伍斌,谢红付,张江林,易梅,李吉..氧化苦参碱对CD4+CD25+调节性T细胞(Tr)和淋巴细胞增殖的影响[J].中国现代医学杂志,2008,18(8):993-999,1004,8.
