中国农业科学(英文版)2009，Vol.8Issue(8)：994-999,6.
Research of Genetic Diversity in Seven Kobresia by AFLP in Tibetan Plateau
Research of Genetic Diversity in Seven Kobresia by AFLP in Tibetan Plateau
摘要
Abstract
This work analyzed the genetic diversity of Kobresia accessions at the molecular level, and further obtained the necessary information for breeding and germplasm evaluation. Genomic DNA of Kobresia was amplified with four E+3 and M+3 primer combinations with AFLP (amplified fragment length polymorphism). AFLP analysis produced 164 scorable bands,of which 154 (93.96%) were polymorphic. The mean Nei's gene diversity index (H) was 0.2430, and the Shannon's information index (I) was 0.4012, indicating the abundant genetic diversity of Kobresia. The 11 Kobresia accessions from Tibetan Plateau, China, can be classified into five groups after cluster analysis based on the UPGMA (unweighted pair group method arithmetic average) method. In general, there was abundant genetic diversity among Kobresia accessions resources, and the genetic coefficient was unrelated to their geographic latitude. Natural habitats influenced genetic differentiation of Kobresia.关键词
Kobresia/genetic diversity/AFLPKey words
Kobresia/genetic diversity/AFLP分类
农业科技引用本文复制引用
ZHENG Hong-mei,HU Tian-ming,WANG Quan-zhen,ZHANG Guo-yun,SONG Jiang-hu..Research of Genetic Diversity in Seven Kobresia by AFLP in Tibetan Plateau[J].中国农业科学(英文版),2009,8(8):994-999,6.基金项目
This work was supported by the Key International Science and Technology Cooperation Program of China (2006DFA33630), and the Key Science and Technology Research Program of Tibet, China (2005011). （2006DFA33630）
