中国农业科学（英文版）2002，Vol.1Issue(9)：998-1005,8.
The Utility of Specific Markers Based on ITS2 Sequences for Molecular Identification and Detection of Trichogramma spp.
The Utility of Specific Markers Based on ITS2 Sequences for Molecular Identification and Detection of Trichogramma spp.
LI Zheng-xi 1SHEN Zuo-rui1
作者信息
- 1. Department of Entomology, China Agricultural University, Beijing 100094, P. R . China
- 折叠
摘要
Abstract
The technology based on specific PCR amplification using internal transcribed spacer 2 of nuclear ribosomal DNA for molecular identification and detection of Trichogramma species was studied. Firstly the ITS2s of six Trichogramma species were cloned and sequenced, and the interspecific sequence variation was analyzed. Secondly the ITS2 regions of six geographical populations of T. dendrolimi were cloned and sequenced, and the intraspecific sequence identity was analyzed. The results show that the interspecific variation and intraspecific similarity of ITS2 sequences are very suitable for designation of specific primers at specieslevel. Screening of specific primers for T. dendrolimi leads to final sensitive and stable diagnostic primers. This system lets non-specialists can not only identify adults (males and females), but also identify eggs in parasitized hosts rapidly and accurately, which is impossible by conventional methods. Further development of this protocol can create a complete set of specific primers for different species of the whole genus Trichogramma.关键词
Specific marker, ITS2-rDNA, Molecular identification, TrichogrammaKey words
Specific marker, ITS2-rDNA, Molecular identification, Trichogramma引用本文复制引用
LI Zheng-xi,SHEN Zuo-rui..The Utility of Specific Markers Based on ITS2 Sequences for Molecular Identification and Detection of Trichogramma spp.[J].中国农业科学（英文版）,2002,1(9):998-1005,8.
