中华医学杂志（英文版）2010，Vol.123Issue(5)：532-536,5.DOI:10.3760/cma.j.issn.0366-6999.2010.05.005
Quercetin in combating H_2O_2 induced early cell apoptosis and mitochondrial damage to normal human keratinocytes
Quercetin in combating H_2O_2 induced early cell apoptosis and mitochondrial damage to normal human keratinocytes
WANG Xiao-yan 1HE Pei-ying 2DU Juan 3ZHANG Jian-zhong1
作者信息
- 1. Department of Dermatology,Peking University People's Hospital,Beijing 100044,China
- 2. Department of Allergology,Beijing Shijitan Hospital,Beijing 100038,China
- 3. Central Laboratory,Peking University People's Hospital,Beijing 100044,China
- 折叠
摘要
Abstract
Background Oxidative stress plays an important role in the pathogenesis of epidermal diseases. This study aimed to investigate the effects of quercetin on the anti-oxidative response and on mitochondrial protection in cultured normal human keratinocytes. Methods Cultured HaCaT cells were treated with different concentrations of H_2O_2 (0, 50, 100, 250, 500 μmol/L) for different periods of time (0.5, 1,2,4 hours) to establish an oxidative stress model. The cultured HaCaT cells were randomly assigned to control, H_2O_2, and quercetin+H_2O_2 groups. For the quercetin groups, the cells were treated with different concentrations of quercetin (0,10, 25, 50 μmol/L) before exposure to H_2O_2. Morphological changes of the cells were observed under an inverted microscope and an electron microscope. The cell viability was detected by the MTT method. The cell apoptosis (AnnexinV/propidium iodide double stain) and mitochondrial membrane potential (△ψm) changes were detected by flow cytometry. Results An oxidative stress model of HaCaT cells was established under a suitable concentration (250 μmol/L) and treated time of H_2O_2 (2 hours). The cell viability and △ψm decreased in a concentration-dependent and time-dependent manner while the percentage of apoptotic cells significantly increased in the H_2O_2 groups compared with the control group (P<0.05). The cell viability and △ψm of the quercetin treated group increased (P<0.05) and the percentage of apoptotic cells decreased at concentrations of 1-50 μmol/L quercetin (P<0.01) compared with H_2O_2 treated group. Conclusion Quercetin can relieve the cell damage and apoptosis from H_2O_2 induced injury to HaCaT cells by anti-oxidation and mitochondrial protection. 关键词
keratinocyte/hydrogen peroxide/quercetin/apoptosis/mitochondria membrane potentialKey words
keratinocyte/hydrogen peroxide/quercetin/apoptosis/mitochondria membrane potential引用本文复制引用
WANG Xiao-yan,HE Pei-ying,DU Juan,ZHANG Jian-zhong..Quercetin in combating H_2O_2 induced early cell apoptosis and mitochondrial damage to normal human keratinocytes [J].中华医学杂志（英文版）,2010,123(5):532-536,5.
