中华医学杂志（英文版）2010，Vol.123Issue(8)：1086-1092,7.DOI:10.3760/cma.j.issn.0366-6999.2010.08.021
SH-SY5Y human neuroblastoma cell line: in vitro cell model of dopaminergic neurons in Parkinson's disease
SH-SY5Y human neuroblastoma cell line: in vitro cell model of dopaminergic neurons in Parkinson's disease
XIE Hong-rong 1HU Lin-sen 1LI Guo-yi1
作者信息
- 1. Department of Neurology,the Firs Hospital,Jilin University,Changchun 130021,Chian
- 折叠
摘要
Abstract
Objective To evaluate the human neurobfastoma SH-SY5Y cell line as an in vitro model of dopaminergic (Daergic) neurons for Parkinson's disease (PD) research and to determine the effect of differentiation on this cell model. Data sources The data of this review were selected from the original reports and reviews related to SH-SY5Y cells published in Chinese and foreign journals (Pubmed 1973 to 2009). Study selection After searching the literature, 60 articles were selected to address this review. Results The SH-SY5Y cell line has become a popular cell model for PD research because this cell line posses many characteristics of Daergic neurons. For example, these cells express tyrosine hydroxylase and dopamine-β-hydroxylase,as well as the dopamine transporter. Moreover, this cell line can be differentiated into a functionally mature neuronal phenotype in the presence of various agents. Upon differentiation, SH-SY5Y cells stop proliferating and a constant cell number is subsequently maintained. However, different differentiating agents induce different neuronal phenotypes and biochemical changes. For example, retinoic acid induces differentiation toward a cholinergic neuronal phenotype and increases the susceptibility of SH-SY5Y cells to neurotoxins and neuroprotective agents, whereas treatment with retinoic acid followed by phorbol ester 12-0-tetradecanoylphorbol-13-acetate results in a Daergic neuronal phenotype and decreases the susceptibility of cells to neurotoxins and neuroprotective agents. Some differentiating agents also alter kinetics of 1-methyl-4-phenyl-pyridinium (MPP~+) uptake, making SH-SY5Y cells more similar to primary mesencephalic neurons.Conclusions Differentiated and undifferentiated SH-SY5Y cells have been widely used as a cell model of Daergic neurons for PD research. Some differentiating agents afford SH-SY5Y cells with more potential for studying neurotoxicity and neuroprotection and are thus more relevant to experimental PD research.关键词
Parkinson's disease/SH-SY5Y cells/model/differentiation/dopaminergic neuronKey words
Parkinson's disease/SH-SY5Y cells/model/differentiation/dopaminergic neuron引用本文复制引用
XIE Hong-rong,HU Lin-sen,LI Guo-yi..SH-SY5Y human neuroblastoma cell line: in vitro cell model of dopaminergic neurons in Parkinson's disease[J].中华医学杂志（英文版）,2010,123(8):1086-1092,7.
