国际医药卫生导报2010，Vol.12Issue(3)：332-335,4.DOI:10.3760/cma.j.issn.1007-1245.2010.03.031
高效液相色谱法测定康艾注射液中氧化苦参碱的含量
Determination of Oxymatrine in Kangai injection by RP-HPLC
黄素培 1乔海灵 2王来海1
作者信息
- 1. 453002,新乡医学院第二附属医院
- 2. 450002,郑州大学临床药理教研室
- 折叠
摘要
Abstract
Objective To establish the HPLC method for oxymatrine analyses in Kangai injection. Method: Diamonsil C_(18)0 column (particle size 5 μm, 200 mm×.6 mm) was used as the stationary phase. The mobile phase was composed as A: (methanol) and B: (ammonium acetate buffer solution) (12.5 mmool/L, 30:. 70, V: V), the flow rate was 1.0 ml·in~(-1), The detection of oxymatrine was performed at wavelength of 210nm and normal temperature. Results Linear range of oxymatrine in the method were 3. 25-120 mg·L~(-1), correlation coefficient is 0.9999; respectively, the minimal concentration of detection of oxymatrine were 0.80 mg·L~(-1). Conclusions A reverse phase high performance liquid chromatographic (RP-HPLC)method for simultaneous determination of oxymatrine in Kangai decoction was established, the method could be used for the analyses of oxymatrine in Kangai injection.
关键词
高效液相色谱法/康艾注射液/氧化苦参碱Key words
HPLC/Kangai injection/Oxymatrine引用本文复制引用
黄素培,乔海灵,王来海..高效液相色谱法测定康艾注射液中氧化苦参碱的含量[J].国际医药卫生导报,2010,12(3):332-335,4.
