国际医药卫生导报2010，Vol.16Issue(17)：2137-2140,4.DOI:10.3760/cma.j.issn.1007-1245.2010.17.027
低压液相层析法与离子交换层析法测定糖化血红蛋白的比较
Low performance liquid chromatography versus ion exchange chromatography for detecting HboA1c
摘要
Abstract
Objective To study the methodological difference between low performance liquid chromatography (LPLC) and ion exchange chromatography (IEC) for detecting HbA1c. Methods We examined the accuracy, precision, interfering factors, and relativity of low performance liquid chromatography and ion exchange chromatography for detecting HbA1c. Results The average CV value for LPLC was 3.01%in batch assay and 3.05% in day to day asay, while that for IEC was 4.93% in batch assay and 5.23% in day to day assay. The average recovery rate was101.8% for LPLC and 107.4% for IEC while the true value of HbA1c was 4.6%, 7.9%, and 12.8%. The relativity of LPLC and IEC was described as the following formulas:Y = 0.982X-0.351, with no statistical differences (P＞0.05). Bilirubin, triglycerides, and cholesterol had no interference for LPLC and IEC. Conclusion LPLC and IEC are simple and sensitive in detecting HbA1c.关键词
糖化血红蛋白/低压液相层析/离子交换层析Key words
HbA1c/Low performance liquid chromatography/Ion exchange chromatography引用本文复制引用
马江涛,陈昕,黄明..低压液相层析法与离子交换层析法测定糖化血红蛋白的比较[J].国际医药卫生导报,2010,16(17):2137-2140,4.
