首页|期刊导航|中华医学杂志（英文版）|Up-regulation of the transient A-type K+ current (IA) in the differentiation of neural stem cells of the early postnatal rat hippocampus
中华医学杂志（英文版）2010，Vol.123Issue(13)：1731-1735,5.DOI:10.3760/cma.j.issn.0366-6999.2010.13.022
Up-regulation of the transient A-type K+ current (IA) in the differentiation of neural stem cells of the early postnatal rat hippocampus
Up-regulation of the transient A-type K+ current (IA) in the differentiation of neural stem cells of the early postnatal rat hippocampus
摘要
Abstract
Background Neural stem cells (NSCs) not only are essential to cell replacement therapy and transplantation in clinical settings, but also provide a unique model for the research into neurogenesis and epigenesis. However, little attention has been paid to the electrophysiological characterization of NSC development. This work aimed to identify whether the morphological neuronal differentiation process in NSCs included changes in the electrophysiological properties of transient A-type K+ currents (IA).Methods NSCs were isolated from early postnatal rat hippocampus and were multiplied in basic serum-free medium containing basic fibroblast growth factor. Potassium currents were investigated and compared using whole-cell patch-clamp techniques and one-way analysis of variance (ANOVA), respectively.Results Compared with NSC-derived neurons, cloned NSCs (cNSCs) had a more positive resting membrane potential, a higher input resistance, and a lower membrane capacitance. Part of cNSCs and NSC-derived neurons possessed both delayed-rectifier K+ currents (IDR) and IA, steady-state activation of IA in cNSCs (half-maximal activation at (21.34±4.37) mV) occurred at a more positive voltage than in NSC-derived neurons at 1-6 days in vitro (half-maximal activation at (12.85±4.19) mV).Conclusions Our research revealed a developmental up-regulation of the IA component during differentiation of postnatal NSCs. Together with the marked developmental up-regulation of IDR in vitro neuronal differentiation we have previously found, the voltage-gated potassium channels may participate in neuronal maturation process.关键词
stem cells/transient A-tyrents/cell differentiation/hippocampus/patch-clamp techniquesKey words
stem cells/transient A-tyrents/cell differentiation/hippocampus/patch-clamp techniques引用本文复制引用
GUO Hong-bo,HUANG Lian-yan,ZOU Yu-xi,ZOU Fei..Up-regulation of the transient A-type K+ current (IA) in the differentiation of neural stem cells of the early postnatal rat hippocampus[J].中华医学杂志（英文版）,2010,123(13):1731-1735,5.基金项目
This work was supported in part by grants from National Natural Science Foundation of China (No.30971183),Guangdong Natural Science Foundation (No.7005213),and Science and Technology Projects in Guangdong Province (No.2009B030801230). （No.30971183）
