国际医药卫生导报2010，Vol.16Issue(20)：2471-2475,5.DOI:10.3760/cma.j.issn.1007-1245.2010.20.010
检测细胞周期分布的改变以探讨温和性高温对鼻咽癌细胞系CNE-2Z放射增敏作用的机制
The mechanism of action of mild high temperature in radiosensitization for nasopharyngeal carcinoma cell line CNE-2Z
摘要
Abstract
Objective To explore the mechanism of action of mild high temperature in radiosensitization for nasopharyngeal carcinoma cell line CNE-2Z. Methods There were control group,heating alone group, radiation alone group, and heating plus radiation group in this study. The change of distribution of G2/M phase and the apoptosis percentage of the cells were analyzed by flow cytometry (FCM).Expressions of CyclinB 1 were detected by Western blot. The morphology of nuclear debris was observed with modified fluorescent staining. Results Greater percentage of distribution of G2/M phase, higher expression of cyclinB1, and more nuclear debris were found in the heating plus radiation group. No percentage of the radiation-induced apoptosis increased. Conclusions Elevated percentage of distribution of G2/M phase and higher expression of CyclinBl is the mechanism of mild high temperature in radiosensitization for nasopharyngeal carcinoma cell line CNE-2Z.关键词
放射增敏/温和性高温/G2/M期/cyclinB1/鼻咽癌细胞系CNE-2ZKey words
Radiosensitization/High temperature/G2/Mphase/CyclinB1/Nasopharyngeal carcinoma cell line CNE-2Z引用本文复制引用
林琼燕..检测细胞周期分布的改变以探讨温和性高温对鼻咽癌细胞系CNE-2Z放射增敏作用的机制[J].国际医药卫生导报,2010,16(20):2471-2475,5.
