国际医药卫生导报2010，Vol.16Issue(16)：2015-2018,4.DOI:10.3760/cma.j.issn.1007-1245.2010.16.032
一株特殊耐药表型肺炎克雷伯菌的耐药机制研究
Resistant mechanisms of a special antibiotic resistance profile of Klebsiella pneumoniae to aminoglycoside
潘树根1
作者信息
- 1. 510150,广州医学院第三附属医院检验科
- 折叠
摘要
Abstract
Objective To investigate the mechanism of a special antibiotic resistance profile of a Klebsiella pneumoniae strain from aminoglycoside resistance to gene patterns. Methods K-B diffusion test, E-test, and three dimensional test were used to investigate the antibiotic resistance profile. AME genes, 16S rRNA methylase genes and integron were confirmed by PCR, respectively. PCR products were then purified and sequenced. Results The special antibiotic resistance profiles were stable.One AME genes [Aac(3)- II ], one 16S rRNA methylase genes (armA), and class 1 integron were identified by PCR. Conclusions The special antibiotic resistance is not induced only by aminoglycoside itself. Aminoglycoside modifying enzymes and plasmid-mediated 16S rRNA methylases may be the major factors which confer aminoglycoside resistance on Klebsiella pneumoniae stains. Until recently, the mechanism causing double inhibition cycles is not clear and needs to be further investigated.关键词
耐药表型/氨基糖苷类抗生素/耐药基因/核糖体16S rRNA/整合子Key words
Antibiotic resistance profile/Aminoglycoside/Gene patterns/16S rRNA/Integron引用本文复制引用
潘树根..一株特殊耐药表型肺炎克雷伯菌的耐药机制研究[J].国际医药卫生导报,2010,16(16):2015-2018,4.
