首页|期刊导航|中国农业科学(英文版)|Analysis of Genetic Diversity in Cultivated and Wild Tomato Varieties in Chinese Market by RAPD and SSR
中国农业科学(英文版)2010,Vol.9Issue(10):1430-1437,8.DOI:10.1016/S1671-2927(09)60234-0
Analysis of Genetic Diversity in Cultivated and Wild Tomato Varieties in Chinese Market by RAPD and SSR
Analysis of Genetic Diversity in Cultivated and Wild Tomato Varieties in Chinese Market by RAPD and SSR
摘要
Abstract
RAPD and SSR were applied to assess genetic diversity in 61 tomato varieties from different species (Solanum lycopersicum L.,hirsutum.Humb L.,pimpinellifolium Miller L.,chilense Dun.L.,chmielenskii L.,peruvianum Miller L.,parvuflorum Miller L.).2062 and 869 clear fragments were amplified by RAPD and SSR,respectively.On the other hand,more polymorphic products were found by SSR as compared to RAPD,i.e.,100 and 43.84%,respectively.In addition,a higher value of the average similarity coefficient and lower PIC value were reflected in RAPD (0.79,0.407) compared to SSR (0.56,0.687).It can be inferred that SSR was a higher effective marker than RAPD to assess genetic diversity in tomato accessions.Similarly,the genetic base of tomato varieties in Chinese market was narrow.It is suggested that wild tomato varieties should be used to enrich the genetic base of the cultivated tomato varieties.关键词
tomato/genetic diversity/RAPD/SSRKey words
tomato/genetic diversity/RAPD/SSR分类
农业科技引用本文复制引用
MENG Fan-juan,XU Xiang-yang,HUANG Feng-lan,LI Jing-fu..Analysis of Genetic Diversity in Cultivated and Wild Tomato Varieties in Chinese Market by RAPD and SSR[J].中国农业科学(英文版),2010,9(10):1430-1437,8.基金项目
This work was supported by the National Natural Science Foundation of China (30901142,30760123),and the Fundamental Research Funds for the Central Universities,China (DL09BA33). (30901142,30760123)