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Analysis of Genetic Diversity in Cultivated and Wild Tomato Varieties in Chinese Market by RAPD and SSR

MENG Fan-juan XU Xiang-yang HUANG Feng-lan LI Jing-fu

中国农业科学(英文版)2010,Vol.9Issue(10):1430-1437,8.
中国农业科学(英文版)2010,Vol.9Issue(10):1430-1437,8.DOI:10.1016/S1671-2927(09)60234-0

Analysis of Genetic Diversity in Cultivated and Wild Tomato Varieties in Chinese Market by RAPD and SSR

Analysis of Genetic Diversity in Cultivated and Wild Tomato Varieties in Chinese Market by RAPD and SSR

MENG Fan-juan 1XU Xiang-yang 2HUANG Feng-lan 1LI Jing-fu3

作者信息

  • 1. College of Life Science,University of Northeast Forestry,Harbin 150040.P.R.China
  • 2. College of Horticulture,Northeast Agricultural University,Harbin 150030,P.R.China
  • 3. College of Life Science,Inner Mongolia University for Nationalities,Tongliao 028000,P.R.China
  • 折叠

摘要

Abstract

RAPD and SSR were applied to assess genetic diversity in 61 tomato varieties from different species (Solanum lycopersicum L.,hirsutum.Humb L.,pimpinellifolium Miller L.,chilense Dun.L.,chmielenskii L.,peruvianum Miller L.,parvuflorum Miller L.).2062 and 869 clear fragments were amplified by RAPD and SSR,respectively.On the other hand,more polymorphic products were found by SSR as compared to RAPD,i.e.,100 and 43.84%,respectively.In addition,a higher value of the average similarity coefficient and lower PIC value were reflected in RAPD (0.79,0.407) compared to SSR (0.56,0.687).It can be inferred that SSR was a higher effective marker than RAPD to assess genetic diversity in tomato accessions.Similarly,the genetic base of tomato varieties in Chinese market was narrow.It is suggested that wild tomato varieties should be used to enrich the genetic base of the cultivated tomato varieties.

关键词

tomato/genetic diversity/RAPD/SSR

Key words

tomato/genetic diversity/RAPD/SSR

分类

农业科技

引用本文复制引用

MENG Fan-juan,XU Xiang-yang,HUANG Feng-lan,LI Jing-fu..Analysis of Genetic Diversity in Cultivated and Wild Tomato Varieties in Chinese Market by RAPD and SSR[J].中国农业科学(英文版),2010,9(10):1430-1437,8.

基金项目

This work was supported by the National Natural Science Foundation of China (30901142,30760123),and the Fundamental Research Funds for the Central Universities,China (DL09BA33). (30901142,30760123)

中国农业科学(英文版)

OACSCD

2095-3119

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