首页|期刊导航|中国神经再生研究（英文版）|Low-frequency electrical stimulation improves neurite outgrowth of dorsal root ganglion neurons in vitro via upregulating Ca2+-mediated brain-derived neurotrophic factor expression
中国神经再生研究（英文版）2010，Vol.5Issue(16)：1256-1260,5.DOI:10.3969/j.issn.1673-5374.2010.16.010
Low-frequency electrical stimulation improves neurite outgrowth of dorsal root ganglion neurons in vitro via upregulating Ca2+-mediated brain-derived neurotrophic factor expression
Low-frequency electrical stimulation improves neurite outgrowth of dorsal root ganglion neurons in vitro via upregulating Ca2+-mediated brain-derived neurotrophic factor expression
摘要
Abstract
Short-term,low-frequency electrical stimulation of neural tissues significantly enhances axonal regeneration of peripheral nerves following injury.However,little is known about the mechanisms of electrical stimulation to induce neurite outgrowth.In the present study,short-term,low-frequency electrical stimulation,using identical stimulation parameters of in vivo experiments,was administered to in vitro dorsal root ganglion(DRG)neurons.Enhanced neurite outgrowth,as well as synthesis and release of brain-derived neurotrophic factor(BDNF),were examined in electrical stimulation-treated DRG neuronal cultures.Because the effects of electrical stimulation on neuronal intracellular signaling molecules are less reported,classic calcium intracellular signals are directly or indirectly involved in electrical stimulation effects on neurons.Cultured DRG neurons were pretreated with the calcium channel blocker nifedipine,followed by electrical stimulation.Results suggested that electrical stimulation not only promoted in vitro neurite outgrowth,but also enhanced BDNF expression.However,nifedipine reduced electrical stimulation-enhanced neurite outgrowth and BDNF biosynthesis.These results suggest that the promoting effects of electrical stimulation on DRG neurite outgrowth could be associated with altered calcium influx,which is involved induction of neuronal BDNF expression and secretion.关键词
electrical stimulation/dorsal root ganglion neurons/neurite outgrowth/brain-derived neurotrophic factor/Ca2+/neural regenerationKey words
electrical stimulation/dorsal root ganglion neurons/neurite outgrowth/brain-derived neurotrophic factor/Ca2+/neural regeneration分类
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Lidan Wan,Rong Xia,Wenlong Ding..Low-frequency electrical stimulation improves neurite outgrowth of dorsal root ganglion neurons in vitro via upregulating Ca2+-mediated brain-derived neurotrophic factor expression[J].中国神经再生研究（英文版）,2010,5(16):1256-1260,5.基金项目
Supported by: the Shanghai Leading Academic Discipline Project,No.S30201 （）
the Doctoral Research Foundation of Nanchang University （）
