首页|期刊导航|中国农业科学（英文版）|Effects of Orexin A on mRNA Expression of Various Neuropeptides in the Hypothalamus and Pituitary,and on Serum LH Levels in Ovariectomized Gilts
中国农业科学（英文版）2010，Vol.9Issue(9)：1362-1371,10.
Effects of Orexin A on mRNA Expression of Various Neuropeptides in the Hypothalamus and Pituitary,and on Serum LH Levels in Ovariectomized Gilts
Effects of Orexin A on mRNA Expression of Various Neuropeptides in the Hypothalamus and Pituitary,and on Serum LH Levels in Ovariectomized Gilts
摘要
Abstract
Orexin has several biological functions,including the regulation of reproductive endocrine signaling,which has received much attention.However,little is known about the mechanism through which orexin regulates the levels of neuroendocrine hormones and peptides.We injected orexin A or physiological saline into the lateral ventricle of 10 ovariectomized(OVX)gilts,and determined the subsequent changes in serum luteinizing hormone(LH)concentration by using radioimmunoassay(RIA).We also examined the expression of GnRH,NPY,and POMC mRNAs in the hypothalamus and that of LH,follicle-stimulating hormone(FSH),POMC,and ghrelin mRNAs in the pituitary by using semi-quantitative reverse transcription polymerase chain reaction.We found the following results:(1)Orexin A transiently promoted LH secretion; serum LH concentration started to increase at 10 min after the orexin injection,peaked at 30 min,and returned to its initial level at1.5 h;(2)orexin A upregulated GnRH mRNA expression and downregulated NPY and POMC mRNAs expression in the hypothalamus;(3)orexin A upregulated LH and FSH mRNAs expression(FSH,P>0.05),but downregulated ghrelin mRNA expression in the pituitary.No significant effects were observed on the pituitary expression of FSH and POMC mRNAs.Our data suggest that orexin A regulates reproductive function by stimulating GnRH and LH release directly and indirectly via its effects on NPY,POMC and ghrelin expression.关键词
orexin A/OVX gilt/relative neuropeptides/LH/hypothalamus/pituitaryKey words
orexin A/OVX gilt/relative neuropeptides/LH/hypothalamus/pituitary分类
农业科技引用本文复制引用
NING Hong-mei,GE Ya-ming,SU Juan,ZHANG Wen-long,YAO Yuan,YANG Gui-hong,LEI Zhi-hai..Effects of Orexin A on mRNA Expression of Various Neuropeptides in the Hypothalamus and Pituitary,and on Serum LH Levels in Ovariectomized Gilts[J].中国农业科学（英文版）,2010,9(9):1362-1371,10.基金项目
This study was sponsored by the National Natural Science Foundation of China(30471248).We thank Prof.Chen Weihua(Nanjing Agricultural University,China)for assistance with the LH assay and Dr.Wang Hongxing(Department of Pathology,Harvard Medical School,USA)for assistance in preparing this manuscript. （30471248）
