首页|期刊导航|中国农业科学（英文版）|Cryopreservation of Queen Honeybee(Apis mellifera camica)Born Worker Eggs by Vitrification
中国农业科学（英文版）2010，Vol.9Issue(9)：1353-1361,9.
Cryopreservation of Queen Honeybee(Apis mellifera camica)Born Worker Eggs by Vitrification
Cryopreservation of Queen Honeybee(Apis mellifera camica)Born Worker Eggs by Vitrification
摘要
Abstract
Many species of insect egg can be targeted individually or(and)collectively for cryopreservation by vitrification.However,there has been no report on cryopreservation of honeybee eggs by vitrification.In an attempt to define a preliminary procedure of cryopreservation of honeybee eggs by vitrification,queen honeybee born worker eggs(worker eggs)were stored through vitrification in liquid nitrogen up to 1 h,and then post-vitrification survival of the vitrified worker eggs in vitro and their hatching rates during maturation in vivo were observed using microscopic and close visual inspections.The procedure of cryopreservation by vitrification included dechorionation with sodium hypochlorite and permeabilization with isopropyl alcohol; equilibration by addition of loading solution(i.e.,25% vitrification storage solution)and dehydration by gradual replacement of loading solution with vitrification storage solution; cooling in liquid nitrogen vapor right before droplet vitrification in liquid nitrogen; and recovery in liquid nitrogen vapor right after storage in liquid nitrogen,thawing at temperature of thawing medium(5% sucrose in TC 100-insect medium)and rehydration by gradual replacement of vitrification storage solution with rehydration solution(5% fetal bovine serum in TC 100-insect medium).It was found that among the worker eggs experiencing cyropreservation by vitrification,1.25% of them were successfully passed through the four life stages,viz.,egg,larva,pupa,and adult.In summary,it can be inferred that although a majority of worker eggs were dead after cyroprescrvation by vitrification,a few of them were developed into larvae,pupae,and finally emerged as adults.关键词
honeybee worker/egg/cryopreservation/vitrificationKey words
honeybee worker/egg/cryopreservation/vitrification分类
农业科技引用本文复制引用
LI Zhi-yong,XUE Yun-bo,WANG Zhi,LI Xing-an..Cryopreservation of Queen Honeybee(Apis mellifera camica)Born Worker Eggs by Vitrification[J].中国农业科学（英文版）,2010,9(9):1353-1361,9.基金项目
We thank Prof.Ge Fengchen,Jilin Provincial Apiculture Institute,China,for his technical assistance.This work was supported by Science Technology Founda-tion of Jilin Province,China(20040555 and 20082215). （20040555 and 20082215）
