首页|期刊导航|中德临床肿瘤学杂志（英文版）|TNF-α induces apoptosis of Molt-4 cells and cell cycle specificity of Bcl-2 phosphyrylation
中德临床肿瘤学杂志（英文版）2010，Vol.9Issue(10)：559-563,5.DOI:10.1007/s10330-010-0681-7
TNF-α induces apoptosis of Molt-4 cells and cell cycle specificity of Bcl-2 phosphyrylation
TNF-α induces apoptosis of Molt-4 cells and cell cycle specificity of Bcl-2 phosphyrylation
摘要
Abstract
Objective:The aim of the study was to observe the expression of Bcl-2 and its phosphorylation in Molt-4 cells induced by tumor necrosis factor-α(TNF-α),and to investigate the possible mechanism of cell cycle specificity of apoptosis.Methods:Exponentially growing Molt-4 cells were treated with TNF-α.Apoptosis was detected by DNA fragmentation assay.API method was applied to illustrate the cell cycle specificity of apoptotic cells.Cells of sub-phases were sorted by FACSvantage flow cytometer and then submitted to immunoblot.Results:Molt-4 cells which were treated with TNF-α went to apoptosis and showed a DNA ladder pattern.Most apoptosis happened in G1-phase of cell cycle.Bcl-2 expression increased for the Molt-4 cells treated with TNF-α.The phosphorylation state of Bcl-2 was only presented in G1-phase cells,in accordance with the specified time and cell cycle phase of apoptosis.Conclusion:The phosphorylation of Bcl-2 in the Molt-4 cells treated with TNF-α happened with the same cell cycle specificity as cell apoptosis.The cell cycle specificity of Bcl-2 phosphorylation was one of the mechanisms of receptor-mediated apoptosis.The cell cycle machine can trigger the apoptosis program.关键词
apoptosis/Bd-2/phosphorylation/cell cycleKey words
apoptosis/Bd-2/phosphorylation/cell cycle分类
医药卫生引用本文复制引用
Changyong Yang,Huijie Zhao,Jianping Gong..TNF-α induces apoptosis of Molt-4 cells and cell cycle specificity of Bcl-2 phosphyrylation[J].中德临床肿瘤学杂志（英文版）,2010,9(10):559-563,5.基金项目
Supported by grants from the Clinical Key Subject Foundation of Health Ministry of China(No.20012537)and National Science and Technology Department"973"Tumor Project(No.2004CB518705). （No.20012537）
